Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Protein thiophosphorylation associated with secretory inhibition in permeabilized chromaffin cells

Journal Article · · Life Sci.; (United States)
;

Permeabilized cells treated with the adenosine triphosphate analog, (/sup 35/S)adenosine-5'-0-3(3-thiotriphosphate) ((..gamma..-/sup 35/S)ATP), showed thiophosphorylation of a small number of cellular proteins. A 54 kilodalton (kDa) protein was heavily thiophosphorylated in unstimulated control cells and a 43 kilodalton protein was more heavily thiophosphorylated in calcium stimulated cells. Intact cells incorporated /sup 35/S into a series of higher molecular weight proteins. Stimulation of prelabelled, permeabilized cells resulted in a loss of /sup 35/S from the cells over a 20 min period. Treatment of permeabilized cells with ATP..gamma..S inhibited secretion and /sup 35/S incorporation into the cells. Pretreatment with ATP..gamma..S resulted in subsequent inhibition of both secretion and the ability of the cells to incorporate /sup 35/S from (..gamma..-/sup 35/S)ATP. These results indicate that the sites normally available for phosphorylation were inactivated by thiophosphorylation and were unavailable to participate in the secretory process. The inhibition of secretion associated with thiophosphorylation of these proteins suggests that they may play a role in the control of secretion by chromaffin cells. 15 references, 1 figure, 3 tables.

Research Organization:
Marquette Univ. School of Dentistry, Milwaukee, WI
OSTI ID:
6138606
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 37:20; ISSN LIFSA
Country of Publication:
United States
Language:
English

Similar Records

Involvement of myosin light-chain kinase in endothelial cell retraction
Journal Article · Sun Dec 31 23:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA) · OSTI ID:6968819
Adenosine 5'-0-(3-thiotriphosphate) and CAMP-dependent protein kinase activity
Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5385069
Selective inhibition of cholinergic receptor-mediated /sup 45/Ca++ uptake and catecholamine secretion from adrenal chromaffin cells by taxol and vinblastine
Journal Article · Mon Oct 01 00:00:00 EDT 1984 · J. Pharmacol. Exp. Ther.; (United States) · OSTI ID:5948032

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DATA
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MOLECULAR WEIGHT
NUCLEI
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PHOSPHORYLATION
PROTEINS
RADIOISOTOPES
SECRETION
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES