Radiation-induced formation of apoptotic bodies in rat thymus

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576309· OSTI ID:6135929

Ohyama, H; Yamada, T; Ohkawa, A; Watanabe, I

The process of interphase death of thymocytes in whole-body X-irradiated rats were studied. Cell size distribution analysis indicates that cell fragments (=apoptotic bodies) appeared in the thymus and increased in number depending on dose (200-1000 R) and time (2-6 hr) after irradiation with corresponding decrease in normal-size thymocytes. Occurrence of nuclear fragmentation in association with the cellular fragmentation was proved with cytofluorometric determination of DNA content in individual cells. Scanning electron microscopic observations also revealed extensive fragmentation of cells in the irradiated rat thymus. The results show clearly that cells as well as nuclei fragments rapidly into smaller pieces of various sizes in the irradiated rat thymus as commonly observed with apoptosis.

• Research Organization: National Inst. of Radiological Sciences, Anagawa, Japan
• OSTI ID: 6135929
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 101:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BODY
CELL FLOW SYSTEMS
CELL KILLING
ELECTROMAGNETIC RADIATION
ELECTRON MICROSCOPY
EXTERNAL IRRADIATION
FRAGMENTATION
IONIZING RADIATIONS
IRRADIATION
LYMPHATIC SYSTEM
MAMMALS
MICROSCOPY
ORGANS
RADIATIONS
RADIOINDUCTION
RATS
RODENTS
SCANNING ELECTRON MICROSCOPY
SOMATIC CELLS
THYMOCYTES
THYMUS
VERTEBRATES
WHOLE-BODY IRRADIATION
X RADIATION