Iron availability in mixed cultures of sulfate-reducing bacteria

Journal Article · Tue Dec 31 23:00:00 EST 1985 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260280114· OSTI ID:6135082

Hauser, J Y; Holder, G A

Postgate's medium C was found to be unsuitable for quantitative studies of mixed microbial cultures containing sulfate-reducing bacteria. Equilibrium calculations of iron concentration, along with the experimental observations show that precipitation of iron occurs in Postgate's medium, despite the presence of citrate and yeast extract. The often ascribed inhibitory effect of sulfide on the growth of sulfate-reducing bacteria may be largely due to the reduced availability of iron, as a result of the precipitation of ferrous sulfide. Because it is essential to clearly define the growth limiting nutrient when carrying out quantitative bacterial growth studies, yeast extract should be excluded from the culture medium. Single-phase, exponential growth was observed for mixed cultures containing sulfate-reducing bacteria, using a chemically defined medium that incorporated the metal complexing agent EDTA. When carrying out quantitative kinetic studies of sulfate-reducing bacteria, a chemically defined medium incorporating EDTA is recommended.

• Research Organization: Monash Univ., Clayton, Australia
• OSTI ID: 6135082
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 28:1; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
BACTERIA
BIOLOGICAL AVAILABILITY
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CHELATING AGENTS
CULTURE MEDIA
EDTA
ELEMENTS
GROWTH
IRON
METALS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
SULFATE-REDUCING BACTERIA
TRANSITION ELEMENTS