Adrenal mediation of ethanol's inhibition of benzo(a)pyrene metabolism. [Rats]

Journal Article · Sun Jan 01 04:00:00 EST 1984 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398409530541· OSTI ID:6133801

Bogdanffy, M S; Schatz, R A; Brown, D R

Previous studies in rats have demonstrated that acute ethanol (1 h) at high doses inhibits xenobiotic metabolism and that the effect is modulated by the adrenals. In this paper, a similar phenomenon is reported for benzo(a)pyrene (BaP) metabolism but the inhibitory effect is restricted to detoxification without effect on activation routes. Rats were administered ethanol (5 g/kg) orally and sacrificed 1 h later. Microsomes were isolated and assayed for capacity to metabolize BaP to activated and detoxified products. Ethanol treatment inhibited detoxification, as evidenced by approximately 50% decrease in 3-hydroxy-BaP formation. There was little effect on metabolic routes forming activated products, as indicated by no change in the rate of dihydrodiol formation. To determine the role of the adrenals in ethanol's inhibitory effect towards detoxication, a similar experiment was performed in adrenalectomized (ADX) rats. ADX alone slightly decreased 3-hydroxy-BaP formation, but treatment with ethanol resulted in no significant differences from ADX controls. Corticosterone administration to ADX rats resulted in an inhibition of the formation of all metabolites. The data suggest that acute ethanol inhibits the detoxication of BaP without effecting activation and that this effect is mediated by the adrenals. This would be expected to increase the proporation of carcinogenic metabolites. 38 references, 2 figures, 3 tables.

• Research Organization: Northeastern Univ., Boston, MA
• OSTI ID: 6133801
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 13:4-6; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL GLANDS
ADRENAL HORMONES
ALCOHOLS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
CELL CONSTITUENTS
CONDENSED AROMATICS
CORTICOSTEROIDS
CORTICOSTERONE
DATA
DETOXIFICATION
ENDOCRINE GLANDS
ETHANOL
EXPERIMENTAL DATA
GLANDS
GLUCOCORTICOIDS
HORMONES
HYDROCARBONS
HYDROXY COMPOUNDS
INFORMATION
INHIBITION
KETONES
METABOLIC ACTIVATION
METABOLISM
METABOLITES
MICROSOMES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PREGNANES
STEROID HORMONES
STEROIDS