Use of uv irradiation to identify the genetic information of vesicular stomatitis virus responsible for shutting off cellular RNA synthesis

Journal Article · Fri Jun 01 04:00:00 EDT 1979 · J. Virol.; (United States)

OSTI ID:6132845

Weck, P K; Carroll, A R; Shattuck, D M; Wagner, R R

UV irradiation of infectious vesicular stomatitis virus was employed to study the relationship between the expression of certain viral gene functions and viral inhibition of RNA synthesis in mouse myeloma (MPC-11) cells. Viral infectivity, protein synthesis, and viral mRNA synthesis were all highly susceptible to inactivation by uv radiation; however, low levels of viral transcriptase activity were detected in vitro in virus preparations subjected to large doses of uv radiation. In sharp contrast, the capacity of vesicular stomatitis virus to shut off cellular transcription was quite resistant to uv radiation. The data presented here indicate that viral transcription is essential to inhibit host RNA metabolism, even though synthesis of viral polypeptides in the inhibited cells could not be detected. At those levels of uv radiation that inactivated all viral gene functions, except viral inhibition of cellular RNA synthesis, the only viral product detected was non-adenylated, low-molecular-weight RNA species.

• Research Organization: Univ. of Virginia School of Medicine, Charlottesville
• OSTI ID: 6132845
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 30:3; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
BIOSYNTHESIS
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GENES
GENETICS
GRAPHS
INFECTIVITY
INFORMATION
MICROORGANISMS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PARASITES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RNA
SYNTHESIS
ULTRAVIOLET RADIATION
VIRUSES