Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Chemical modification studies of the streptokinase-plasminogen interaction

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6126182
;
The streptokinase (SK) interaction with human plasminogen (Pg) was investigated by differential chemical modification. In separate experiments, available lysine residues in both free streptokinase and streptokinase in complex with Pg were trace labeled by reaction with high specific activity (/sup 3/H)-acetic anhydride at a reagent-to-lysine molar ratio of 0.5%. The (/sup 3/H)-acetyl-SK from the complex was reisolated. Both the free and complex forms of /sup 3/H-acetyl-SK were then each mixed with uniformly and quantitatively modified (/sup 14/C)-acetyl-SK in a /sup 3/H//sup 14/C isotopic ratio of ten-to-one. Each of the SK forms was fragmented by reaction with CNBr. These CNBr fragments, which were purified by Sephadex G-75 chromatography, were further cleaved by proteases to produce peptides containing a minimum number of lysines. After the isolation of peptides by reversed-phase HPLC, the /sup 3/H//sup 14/C ratios of lysines were individually determined. By comparison of the /sup 3/H//sup 14/C ratio's in the free-SK and complex-SK it was found that the majority of lysines did not change its reactivities in free or complex forms. However, several lysines were relatively unreactive in the SK complexed with Pg as compared to that in free SK. This suggests that these residues are either in the complex binding interface or they were less reactive as a result of conformational change induced by complex formation.
Research Organization:
Oklahoma Medical Research Foundation, Oklahoma City
OSTI ID:
6126182
Report Number(s):
CONF-870644-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
Country of Publication:
United States
Language:
English

Similar Records

Catabolism of streptokinase and polyethylene glycol-streptokinase: Evidence for transport of intact forms through the biliary system in the mouse
Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Blood (Journal of Hematology); (USA) · OSTI ID:6873828
Binding site on Rhodospirillum rubrum cytochrome c/sub 2/ for the Rhodospirillum rubrum cytochrome bc/sub 1/ complex
Journal Article · Mon Nov 30 23:00:00 EST 1987 · Biochemistry; (United States) · OSTI ID:5446088
Trapping and partial characterization of an adduct postulated to be the covalent catalytic ternary complex of thymidylate synthetase
Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5151248

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
COMPLEXES
DRUGS
ENZYMES
FIBRINOLYTIC AGENTS
HEMATOLOGIC AGENTS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPE RATIO
KINETICS
LABELLED COMPOUNDS
LYSINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PLASMINOGEN
REACTION KINETICS
SEPARATION PROCESSES
SH-PROTEINASES
STREPTOCOCCAL PROTEINASE
TRACER TECHNIQUES
TRITIUM COMPOUNDS