Phosphorylation of terminal deoxynucleotidyl transferase in leukemic cells
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
- Univ. of New Mexico School of Medicine, Albuquerque
Phosphorylation of terminal deoxynucleotidyl transferase within leukemic cells has been demonstrated, using /sup 32/P labelling of intact cells in culture, followed by immunoprecipitation of the cellular extracts using an anti-terminal transferase antiserum. The phosphate linkage was found to involve serine and threonine residues. Purified calf thymus terminal transferase served as a substrate for cyclic AMP independent protein kinase obtained from leukemic cells. Phosphorylation in vitro of terminal transferase was accompanied by increased activity and decreased inhibition by excess ribo-ATP. These results indicate that terminal transferase is a physiologic cyclic AMP independent protein kinase substrate, and that this reaction may be important in its control.
- OSTI ID:
- 6123887
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 106:2; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
IN VITRO
ISOTOPES
KINETICS
LABELLING
LEUKEMIA VIRUSES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
RADIOISOTOPES
REACTION KINETICS
TRANSFERASES
TUMOR CELLS
VIRUSES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
IN VITRO
ISOTOPES
KINETICS
LABELLING
LEUKEMIA VIRUSES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
RADIOISOTOPES
REACTION KINETICS
TRANSFERASES
TUMOR CELLS
VIRUSES