Attogram detection limit for aqueous dye samples by laser-induced fluorescence

Journal Article · Fri Feb 18 00:00:00 EST 1983 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.6823553· OSTI ID:6109030

Dovichi, N J; Martin, J C; Keller, R A

A modified flow cytometer has been used to detect attogram quantities of aqueous rhodamine 6G by laser-induced fluorescence analysis. A detection limit of 28 attograms (35,000 molecules) was obtained, nearly two orders of magnitude better than earlier measurements. The detection limit in concentration units was 1.4 x 10/sup -13/ mole per liter. During the 1-second measurement period, the total volume sampled was 0.42 microliter. On average, only half a rhodamine 6G molecule was present in the 6-picoliter probed volume.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 6109030
• Journal Information: Science (Washington, D.C.); (United States), Vol. 219
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
RHODAMINES
FLUORESCENCE SPECTROSCOPY
LASER SPECTROSCOPY
ACCURACY
AQUEOUS SOLUTIONS
CALIBRATION
CELL FLOW SYSTEMS
COMPARATIVE EVALUATIONS
FLOW RATE
QUANTITY RATIO
SENSITIVITY
SPECIFICATIONS
SPECTRAL RESPONSE
AMINES
CARBOXYLIC ACIDS
DISPERSIONS
DYES
EMISSION SPECTROSCOPY
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MIXTURES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
REAGENTS
SOLUTIONS
SPECTROSCOPY
400104* - Spectral Procedures- (-1987)