Cytopathogenicity of Naegleria for cultured neuroblastoma cells

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:6107696

Fulford, D E

The cytopathic activity of live Naegleria amoebae and cell-free lysates of Naegleria for B-103 rat neuroblastoma cells was investigated using a /sup 51/Cr release assay. Live amoebae and cell-free lysates of N. fowleri, N. australiensis, N. lovaniensis, and N. gruberi all induced sufficient damage to radiolabeled B-103 cells to cause a significant release of chromium. The cytotoxic activity present in the cell-free lysates of N. fowleri can be recovered in the supernatant fluid following centrifugation at 100,000xg and precipitation of the 100,000xg supernatant fluid with ammonium sulfate. Initial characterization of the cytotoxic factor indicates that it is a heat labile, pH sensitive, soluble protein. The cytotoxic activity is abolished by either extraction, unaffected by repeated freeze-thawing, and is not sensitive to inhibitors of proteolytic enzymes. Phospholipase A activity was detected in the cytotoxic ammonium sulfate precipitable material, suggesting that this enzyme activity may have a role in the cytotoxic activity of the cell-free lysates.

• Research Organization: Virginia Commonwealth Univ., Richmond (USA). School of Medicine
• OSTI ID: 6107696
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
AMOEBA
TOXICITY
TUMOR CELLS
RADIOASSAY
CHROMIUM 51
ENZYME ACTIVITY
PATHOGENESIS
RATS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
CHROMIUM ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
ISOTOPES
MAMMALS
MICROORGANISMS
NUCLEI
PROTOZOA
RADIOISOTOPES
RODENTS
SARCODINA
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics