Genetic and biochemical characterization of the thymidine kinase gene from herpesvirus of turkeys

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · J. Virol.; (United States)

OSTI ID:6102475

Martin, S L; Aparisio, D I; Bandyopadhyay, P K

The thymidine kinase gene encoded by herpesvirus of turkeys has been identified and characterized. A viral mutant (ATR/sup 0/) resistant to 1-..beta..-D-arabinofuranosylthymine was isolated. This mutant was also resistant to 1-(2-fluoro-2-deoxy-..beta..-D-arabinofuronosyl)-5-methyluracil and was unable to incorporate (/sup 125/I)deoxycytidine into DNA. The mutant phenotype was rescued by a cloned region of the turkey herpesvirus genome whose DNA sequence was found to contain an open reading frame similar to that for known thymidine kinases from other viruses. When expressed in Escherichia coli, this open reading frame complemented a thymidine kinase-deficient strain and resulted in thymidine kinase activity in extracts assayed in vitro.

• Research Organization: Synergen, Inc., Boulder, CO (USA)
• OSTI ID: 6102475
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 63:6; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
AZINES
BACTERIA
BETA DECAY RADIOISOTOPES
BIRDS
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
FOWL
GENE REGULATION
GENES
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOTYPE
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
THYMIDINE
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES
VIRAL DISEASES