Micro-PIGE determination of fluorine distribution in developing hamster tooth germs

Journal Article · Mon May 01 04:00:00 EDT 1989 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/37.5.2703697· OSTI ID:6102461

Lyaruu, D M; Lenglet, W J; Woeltgens, J H.B.; Bronckers, A L

A micro-PIGE (Proton-Induced gamma-ray Emission) technique based on the delayed 5/2+----1/2+ nuclear transition of fluorine (E gamma = 197 keV, t1/2 = 87 ns) emitted after /sup 19/F(p,p', gamma)/sup 19/F reaction was used to detect and study the distribution of fluorine in the developing enamel organ during pre-eruptive stages, i.e., the transitional to early maturation stages of enamel formation in neonatal hamsters administered a single IP dose of sodium fluoride (20 mg NaF/kg body weight). The aforementioned nuclear reaction is unique for fluorine, and therefore detection of gamma-rays emanating from this reaction in a biological specimen implies a positive identification of fluorine at that particular site. Calcium and phosphorus X-rays were also recorded and used as parameters for assessment of the relationship between the degree of mineralization and fluoride incorporation into the enamel organ. The highest fluorine concentration in the enamel organ was recorded in the dentin near the dentin-enamel junction (DEJ). In the enamel, the highest concentration of fluorine was found to be associated with the more mature areas of the enamel near the DEJ, but gradually decreased in the direction of the enamel surface. Fluorine was not detected in the control germs. These results suggest that administration of fluoride in high doses during the pre-eruptive stages of enamel formation leads to incorporation of the ion into the forming dentin and enamel mineral, and that the enamel matrix does not seem to bind fluoride avidly.

• Research Organization: Vrije Universiteit, Amsterdam (Netherlands)
• OSTI ID: 6102461
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 37:5; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ANIMALS
CHEMICAL ANALYSIS
COATINGS
DENTIN
DENTISTRY
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTROMAGNETIC RADIATION
ENAMELS
FLUORIDES
FLUORINE 19
FLUORINE COMPOUNDS
FLUORINE ISOTOPES
GAMMA RADIATION
HALIDES
HALOGEN COMPOUNDS
HAMSTERS
IONIZING RADIATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEDICINE
NONDESTRUCTIVE ANALYSIS
NUCLEI
ODD-EVEN NUCLEI
ORAL CAVITY
RADIATIONS
RODENTS
SODIUM COMPOUNDS
SODIUM FLUORIDES
STABLE ISOTOPES
TEETH
TISSUE DISTRIBUTION
VERTEBRATES
X-RAY EMISSION ANALYSIS