Characterization of beta adrenoceptors on cultured endothelial cells by radioligand binding

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90276-W· OSTI ID:6102016

Ahmad, S; Chretien, P; Shen, S H ^[1]; Daniel, E E ^[2]

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1. National Research Council of Canada, Montreal, Quebec (Canada)
2. McMaster Univ., Hamilton, Ontario (Canada)

The properties of {beta}-adrenoceptors present on the cultured bovine pulmonary artery endothelial cells were studied by radioligand binding. These cells contain a high density of {beta}-adrenoceptors ({approximately} 16,000 receptors/cell) having high affinity (Kd 18 pM) for {sup 125}I-iodocyanopindolol (ICYP). Competition binding experiments suggested the presence of more than two subtype of {beta}-adrenoceptors. 25% of the total population of receptors was found to be of {beta}{sub 1}-type. The remaining 75% represented a mixed population containing what is suggested to be a mixture of {beta}{sub 2}- and atypical {beta}-adrenoceptors.

• OSTI ID: 6102016
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 47:25; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CHEMICAL COMPOSITION
CPB
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SYMPATHOMIMETICS
TRACER TECHNIQUES