Occurrence of lipid A variants with 27-hydroxyoctacosanoic acid in lipopolysaccharides from members of the family Rhizobiaceae
- Univ. of Georgia, Athens (United States)
- Max-Planck Inst. fuer Immunbiologie, Freiburg-Zaehringen (West Germany)
- Inst. for Fermentation, Osaka (Japan)
- Michigan State Univ., East Lansing (United States)
Lipopolysaccharides (LPSs) isolated from several strains of Rhizobium, Bradyrhizobium, Agrobacterium, and Azorhizobium were screened for the presence of 27-hydroxyoctacosanoic acid. The LPSs from all strains, with the exception of Azorhizobium caulinodans, contained various amounts of this long-chain hydroxy fatty acid in the lipid A fractions. Analysis of the lipid A sugars revealed three types of backbones: those containing glucosamine (as found in Rhizobium meliloti and Thizobium fredii), those containing glucosamine and galacturonic acid (as found in Rhizobium leguminosarum bv. phaseoli, trifolii, and viciae), and those containing clucosamine and galacturonic acid (as found in Rhizobium leguminosarum bv. phaseoli, trifolii, and viciae), and those containing 2,3-diamino-2,3-dideoxyglucose either alone or in combination with glucosamine (as found in Bradyrhizobium japonicum and Bradyrhizobium sp. (Lupinus) strain DSM 30140). The distribution of 27-hydroxyoctacosamoic acid as well as analysis of lipid A backbone sugars revealed the taxonomic relatedness of various strains of the Rhizobiaceae.
- DOE Contract Number:
- FG09-87ER13810
- OSTI ID:
- 6101287
- Journal Information:
- Journal of Bacteriology; (United States), Vol. 173:7; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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