Synthesis of tritium-labeled 14 alpha-methyl-5 alpha-cholest-7-en-3 beta-ol and its enzymatic demethylation
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:6101234
14 alpha-Methyl-5 alpha-cholest-7-en-3-one has been prepared and labeled with tritium by isotope exchange chromatography. Reduction with lithium aluminum hydride furnished pure, highly labeled 14 alpha-methyl-5 alpha-cholest-7-en-3 beta-ol. Incubation of this compound with homogenates of rat liver results in its conversion to cholesterol, which has been identified by a variety of chemical and chromatographic techniques. The results show that enzymatic removal of the 14 alpha-methyl group from synthetic compounds is feasible, and offer a new approach to the study of the demethylation mechanism in steroid structures.
- OSTI ID:
- 6101234
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 241:7; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
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5 alpha-Cholest-8(14)-en-3 beta-ol-15-one. In vivo conversion to cholesterol upon oral administration to a nonhuman primate
Thesis/Dissertation
·
Sat Dec 31 23:00:00 EST 1988
·
OSTI ID:5475602
Identification of ergosterol and inhibition of sterol synthesis by. Delta. sup 5 -sterols in GL7, an auxotrophic mutant of yeast
Thesis/Dissertation
·
Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:5264038
5 alpha-Cholest-8(14)-en-3 beta-ol-15-one. In vivo conversion to cholesterol upon oral administration to a nonhuman primate
Journal Article
·
Fri Aug 14 00:00:00 EDT 1987
· Biochem. Biophys. Res. Commun.; (United States)
·
OSTI ID:6090044
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOSYNTHESIS
BODY
CHEMICAL COMPOSITION
CHOLESTEROL
CHROMATOGRAPHY
DIGESTIVE SYSTEM
GLANDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPIC EXCHANGE
LABELLED COMPOUNDS
LABELLING
LIVER
MAMMALS
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
SEPARATION PROCESSES
STEROIDS
STEROLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOSYNTHESIS
BODY
CHEMICAL COMPOSITION
CHOLESTEROL
CHROMATOGRAPHY
DIGESTIVE SYSTEM
GLANDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPIC EXCHANGE
LABELLED COMPOUNDS
LABELLING
LIVER
MAMMALS
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
SEPARATION PROCESSES
STEROIDS
STEROLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES