Metabolic regulation during early frog development: glycogenic flux in Xenopus oocytes, eggs, and embryos

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Dev. Biol.; (United States)

DOI:https://doi.org/10.1016/0012-1606(89)90246-7· OSTI ID:6101068

Dworkin, M B; Dworkin-Rastl, E

/sup 32/P-labeled glucose 6-phosphate and phosphoenolpyruvate were injected into oocytes, fertilized eggs, and early embryos of Xenopus laevis, and the /sup 32/P label was followed into glycolytic enzymes and acid-soluble metabolites. The kinetics of labeling of phosphoglucomutase and phosphoglyceromutase and the formation of specific metabolites were used to measure carbon flux through glycolytic intermediates in these cells. In full-grown stage VI oocytes, fertilized eggs, and cells of cleaving embryos, carbon metabolism is in the glycogenic direction. Glycolytic intermediates injected into these cells were metabolized into UDP-glucose and then presumably into glycogen. Carbon flow between phosphoenolpyruvate and glucose 6-phosphate does not utilize fructose 1,6-bisphosphatase; rather, it may depend largely on enzymes of the pentose phosphate pathway. Maturation and fertilization of the oocyte did not result in a change in the qualitative pattern of metabolites formed. Pyruvate kinase, although abundant in oocytes and embryos, is essentially inactive in these cells. Pyruvate kinase also appears to be inactive in small previtellogenic stage II oocytes; however, in these cells injected glycolytic intermediates were not metabolized to UDP-glucose.

• Research Organization: Ernst Boehringer Institut, Vienna (Austria)
• OSTI ID: 6101068
• Journal Information: Dev. Biol.; (United States), Journal Name: Dev. Biol.; (United States) Vol. 132:2; ISSN DEBIA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOHYDRATES
CARBON
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYMES
FROGS
GERM CELLS
GLUCOSE
GLYCOGEN
GLYCOLYSIS
HEXOSES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
METABOLISM
METABOLITES
MONOSACCHARIDES
NONMETALS
NUCLEI
ODD-ODD NUCLEI
ONTOGENESIS
OOCYTES
ORGANIC COMPOUNDS
PHOSPHOENOLPYRUVATE
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES