(Anaerobic metabolism of aromatic compounds by phototrophic bacteria: Biochemical aspects)

Technical Report · Mon Jan 01 04:00:00 EST 1990

OSTI ID:6098853

Gibson, J

Major effort has gone into purifying and characterizing a second Coenzyme A ligase involved in anaerobic aromatic acid metabolism in Rhodopseudomonas palustris. This enzyme has some overlap in specificity with the benzoate-CoA ligase previously characterized, but differs in many other characteristics, and is antigenically unrelated. Some amino acid sequence information has been obtained, and this as well as a highly specific antiserum, will be useful for cloning the structural gene for the new ligase. In collaboration with Dr. Harwood, at the University of Iowa, we have continued to characterize a group of mutants obtained after Tn5 insertion into our clone bank, followed by transfer of the entire mutagenized bank into wild-type R. palustris. A clone which complements a group of these mutations has been insolated, and is currently being tested for ability to restore anaerobic growth on aromatic acids to other mutants isolated after chemical or uv mutagenesis of the wild-type strain. The close ties with Dr. Harwood's lab were demonstrated by the week-long visit of one of her students during last summer, to learn how to purify the benzoate CoA ligase from R. palustris. A search for possible intermediates in anaerobic metabolism of benzoyl CoA has been initiated in collaboration with Dr. Gibson, focussing on the possible role 1,4-dihydrobenzoyl CoA as a first reduction product.

• Research Organization: Cornell Univ., Ithaca, NY (USA). Dept. of Microbiology
• Sponsoring Organization: DOE; USDOE, Washington, DC (USA)
• DOE Contract Number: FG02-86ER13495
• OSTI ID: 6098853
• Report Number(s): DOE/ER/13495-T4; ON: DE91009799
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANAEROBIC CONDITIONS
BENZOIC ACID
CARBOXYLIC ACIDS
CHEMICAL COMPOSITION
CLONING
DOCUMENT TYPES
ENZYMES
FRACTIONATION
GROWTH
LIGASES
METABOLISM
MOLECULAR STRUCTURE
MONOCARBOXYLIC ACIDS
MUTANTS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOTOSYNTHETIC BACTERIA
PROGRESS REPORT
SEPARATION PROCESSES