Neutron scatter studies of chromatin structures related to function

Technical Report · Mon Jan 01 04:00:00 EST 1990

OSTI ID:6098814

In the Progress Report for last year (7-1-88 to 6-30-89) we proposed to complete the following experiments: (1) Structure of TFIIIA/DNA complex, (2) Effect of histone acetylation on nucleosome structure, and (3) Location of lysine rich histone H5 on the nucleosome. Our major source of neutrons is LANSCE, LANL. However, for the period of this report LANSCE has been down between cycles of operation. Continuing neutron scatter studies have been carried out at the Institute Laue Langevin, Grenoble, France, on the trimmed nucleosome core particles. X-ray scatter studies have been carried out at DESY, Hamburg on the histone octamer and trimmed octamer. X-ray scatter studies have been performed also at LANL on proposed objectives. We have continued with the following of our research program; (i) assembly of fully characterized nucleosomes; (ii) effect of histone acetylation on nucleosomes; (iii) effect of full acetylation of H3 and H4 on nucleosome DNA linking number; (iv) assembly and characterization of defined minichromosomes; (v) neutron and X-ray scatter of the histone octamer and trimmed octamer; (vi) structural studies of human sperm chromatin, histones and protamines. 5 refs.

• Research Organization: California Univ., Davis, CA (USA). Dept. of Physics
• Sponsoring Organization: DOE; USDOE, Washington, DC (USA)
• DOE Contract Number: FG03-88ER60673
• OSTI ID: 6098814
• Report Number(s): DOE/ER/60673-T1; ON: DE91009837
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400101 -- Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACETYLATION
ACYLATION
BARYON REACTIONS
CHEMICAL REACTIONS
CHROMATIN
COAGULANTS
DOCUMENT TYPES
DRUGS
ELECTROMAGNETIC RADIATION
GAMETES
GERM CELLS
HADRON REACTIONS
HEMATOLOGIC AGENTS
HEPARIN ANTAGONISTS
HISTONES
IONIZING RADIATIONS
MOLECULAR STRUCTURE
NEUTRON REACTIONS
NUCLEAR REACTIONS
NUCLEON REACTIONS
NUCLEOPROTEINS
NUCLEOSOMES
ORGANIC COMPOUNDS
PROGRESS REPORT
PROTAMINES
PROTEIN STRUCTURE
PROTEINS
RADIATIONS
RESEARCH PROGRAMS
SCATTERING
SPERMATOZOA
STRUCTURE-ACTIVITY RELATIONSHIPS
X RADIATION