Induced resistance to platinum in Chinese hamster ovary cells

Journal Article · Sun Jan 01 04:00:00 EST 1984 · J. Environ. Sci. Health, Part A; (United States)

DOI:https://doi.org/10.1080/10934528409375157· OSTI ID:6098796

Smith, B L; Hanna, M L; Taylor, R T

Cellular resistances to the toxic effects of two environmentally and industrially important platinum (Pt) complexes (K/sub 2/PtCl/sub 6/ and Pt(SO/sub 4/)/sub 2/) were induced separately in Chinese hamster ovary cells (CHO-S) by continuous exposure to the compounds for 5 and 4 months, respectively. The two resulting cell lines, 220/sup R/ (resistant to 220 ..mu..M K/sub 2/PtCl/sub 6/) and 550/sup R/ (resistant to 550 ..mu..M Pt(SO/sub 4/)/sub 2/) have resistant phenotypes which are stable for at least 55 population doublings in the absence of a selective agent (i.e., Pt). Each resistance is quite specific with respect to the Pt atom as well as the attached ligands, but both cell lines show some cross resistance to other Pt complexes and to one other Noble metal complex (K/sub 3/RhCl/sub 6/). X-Ray fluorescence determinations of Pt in parental CHO-S and 220/sup R/ cells cultured in the presence and absence of K/sub 2/PtCl/sub 6/ demonstrated that very little of this compound is taken up by either cell line. However, comparable analyses revealed that the amount of Pt accumulated by 550/sup R/ cells grown in Pt(SO/sub 4/)/sub 2/ is 3-4 times the amount taken up by the parental CHO-S cells. Cell fusions between each resistant cell line and the Pt-sensitive CHO-S line yielded hybrids which, in each case, display Pt resistances that are intermediate between those of the resistant and sensitive cells employed in the hybridizations. The resistant phenotypes are therefore codominantly regulated. 33 references, 7 figures, 4 tables.

• Research Organization: Lawrence Livermore National Lab., CA
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6098796
• Journal Information: J. Environ. Sci. Health, Part A; (United States), Journal Name: J. Environ. Sci. Health, Part A; (United States) Vol. A19:3; ISSN JESED
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
CHEMICAL ANALYSIS
CHO CELLS
COMPLEXES
CONTROL
GENETIC CONTROL
HYBRIDIZATION
NONDESTRUCTIVE ANALYSIS
PEST CONTROL
PHENOTYPE
PLATINUM COMPLEXES
SENSITIVITY
TOLERANCE
TOXICITY
TRANSITION ELEMENT COMPLEXES
X-RAY EMISSION ANALYSIS
X-RAY FLUORESCENCE ANALYSIS