Lack of biological activity of preproendothelin (110-130) in several endothelin assays

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90308-E· OSTI ID:6098660

Cade, C; Lumma, Jr, W C; Mohan, R; Rubanyi, G M; Parker-Botelho, L H ^[1]

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1. Berlex Labs., Inc., Cedar Knolls, NJ (USA)

A 21 amino acid peptide containing the prepropendothelin sequence from amino acids 110 to 130 and two intrachain disulfide bonds was synthesized and tested for biological activity in the following endothelin assays: (1) a competition binding assay using ({sup 125}I)ET-1 and dog heart membranes, (2) three RIA's using {sup 125}-ET-1, -2 and -3 and the respective anti-ET rabbit antisera; and (3) a contractile activity bioassay using hamster aortic rings. The synthetic peptide which has been referred to as the endothelin-like peptide occurs 36 amino acids C-terminal to endothelin in the prepro-protein sequence. It contains only 40% sequence homology to the three endothelin isoforms, but has the same sequence and cyclization pattern of cysteines at positions 1, 3, 11 and 15. Despite the overall similarity in secondary structure to the three isoforms of endothelin and sarafotoxin S6b, preproendothelin (110-130) had no activity in any of the assays when tested at concentrations of 10{sup {minus}10}M to 10{sup {minus}5}M.

• OSTI ID: 6098660
• Journal Information: Life Sciences; (USA), Vol. 47:23; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PEPTIDES
RADIOIMMUNOASSAY
AMINO ACID SEQUENCE
CELL MEMBRANES
DOGS
HAMSTERS
HEART
IODINE 125
MOLECULAR STRUCTURE
RABBITS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
ELECTRON CAPTURE RADIOISOTOPES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques