Functional complementation of ataxia-telangiectasia group D (AT-D) cells by microcell-mediated chromosome transfer and mapping of the AT-D locus to the region 11q22-23

Journal Article · Mon Jul 01 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.13.5907· OSTI ID:6096756

Lambert, C; Donlon, T; Friedberg, E C ^[1]; Schultz, R A; McDaniel, L D ^[2]; Smith, M; Wagner-McPherson, C; Stanbridge, E J ^[3]

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1. Stanford Univ. School of Medicine, CA (United States)
2. Univ. of Maryland, Baltimore (United States)
3. Univ. of California, Irvine (United States)

The hereditary human disease ataxia-telangiectasia (AT) is characterized by phenotypic complexity at the cellular level. The authors show that multiple mutant phenotypes of immortalized AT cells from genetic complementation group D (AT-D) are corrected after the introduction of a single human chromosome from a human-mouse hybrid line by microcell-mediated chromosome transfer. This chromosome is cytogenetically abnormal. It consists primarily of human chromosome 18, but it carries translocated material from the region 11q22-23, where one or more AT genes have been previously mapped by linkage analysis. A cytogenetically normal human chromosome 18 does not complement AT-D cells after microcell-mediated transfer, whereas a normal human chromosome 11 does. They conclude that the AT-D gene is located on chromosome 11q22-23.

• DOE Contract Number: FG03-88ER60676
• OSTI ID: 6096756
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:13; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CYCLE
CESIUM 137
CESIUM ISOTOPES
CHROMOSOMES
COMPLEMENT
DISEASES
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ETIOLOGY
GAMMA RADIATION
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HETEROCHROMOSOMES
INTERMEDIATE MASS NUCLEI
IONIZING RADIATIONS
ISOTOPES
MAPPING
NUCLEI
NUCLEIC ACID REPLICATION
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHENOTYPE
PROTEINS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY
SKIN DISEASES
SOMATIC CELLS
SURVIVAL CURVES
TELANGIECTASIS
VASCULAR DISEASES
YEARS LIVING RADIOISOTOPES