Formation of intermediate cementum. III: /sup 3/H-tryptophan and /sup 3/H-proline uptake into the epithelial root sheath of Hertwig in vitro

Journal Article · Fri Jan 01 04:00:00 EST 1982 · J. Craniofacial Genet. Dev. Biol.; (United States)

OSTI ID:6096408

Lindskog, S; Hammarstroem, L

The intermediate cementum is a narrow, mineralized tissue between the cementum and dentin. Recent studies have shown that this tissue is mineralized by the epithelial root sheath in a way similar to the mineralization of the innermost layer of aprismatic enamel. In the present investigation uptake of proline and tryptophan into the epithelial root sheath was studied with autoradiography. Tryptophan is an amino acid that is incorporated into enamel matrix but not into collagen. Tryptophan uptake was significant in the whole epithelial root sheath, but not into the odontoblasts or predentin. Proline was incorporated into the predentin while the root sheath was unlabeled. This indicated that the matrix of the intermediate cementum was formed by the epithelial root sheath of Hertwig, and that this matrix was a noncollagenous matrix possibly of the same nature as enamel matrix.

• Research Organization: Department of Oral Pathology, School of Dentistry, Karolinska Institutet, Stockholm, Sweden
• OSTI ID: 6096408
• Journal Information: J. Craniofacial Genet. Dev. Biol.; (United States), Journal Name: J. Craniofacial Genet. Dev. Biol.; (United States) Vol. 2:2; ISSN JCGBD
• Country of Publication: United States
• Language: English

Related Subjects

550801* -- Morphology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL TISSUES
AUTORADIOGRAPHY
AZOLES
BODY
BONE TISSUES
CARBOXYLIC ACIDS
COATINGS
CONNECTIVE TISSUE
DIGESTIVE SYSTEM
ENAMELS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INDOLES
LABELLED COMPOUNDS
MINERALIZATION
MORPHOLOGY
ORAL CAVITY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROLINE
PYRROLES
PYRROLIDINES
TEETH
TISSUES
TRITIUM COMPOUNDS
TRYPTOPHAN