Repair and cell cycle response in cells exposed to environmental biohazards. Progress report, October 1, 1976--May 31, 1979

Technical Report · Mon Jan 01 00:00:00 EST 1979

OSTI ID:6095570

Billen, D

A wide variety of environmental agents are known which induce damage in DNA leading to an inhibition of DNA synthesis or faulty replication. Both results may cause cell death or mutation. Both bacteria and mommalian cells are being used to assess the roles of the several known DNA polymerases and other DNA metabolic enzymes and factors in DNA repair, replication and recombination. The many DNA mutants of E. coli and B. subtilis provide a genetic approach to measuring the role of individual components of the DNA repair and replicative system. Because of the advantage of controlling pools and precursors of nucleic acid synthesis we have further developed the use of permeabilized cells for such studies. In addition a series of repair studies with Bacillus subtilis have been carried on because of the unique genetic manipulation of this system which includes the ability of cells to be easily transformed by exogenous DNA. The information obtained with prokaryotes provides leads to assess the details of DNA repair and replication in mammalian systems including man.

• Research Organization: Tennessee Univ., Knoxville (USA); Oak Ridge National Lab., TN (USA)
• DOE Contract Number: EY-76-S-05-4568
• OSTI ID: 6095570
• Report Number(s): ORO-4568-20; TRN: 79-018693
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL REPAIR
MUTAGENESIS
BACTERIA
BIOLOGICAL RADIATION EFFECTS
CELL CYCLE
DNA
MAMMALS
ULTRAVIOLET RADIATION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
ELECTROMAGNETIC RADIATION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
VERTEBRATES
560152* - Radiation Effects on Animals- Animals