Characterization of a dopamine receptor (DA sub 2K ) in the kidney inner medulla

Journal Article · Mon Apr 15 04:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.8.3170· OSTI ID:6095185

Huo, T; Ye, M Q; Healy, D P ^[1]

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1. City Univ. of New York, NY (United States)

Dopamine (DA) produces a natriuretic/diuretic response in the kidney by mechanisms that are still not well understood. There is some indication that DA{sub 2} receptors may be involved in mediating the effects of DA, but little is known regarding the nature of this receptor in the kidney. Autoradiographic localization of ({sup 3}H)spiperone, a DA{sub 2} antagonist, indicated that high-density binding was restricted to inner medullary collecting ducts (IMCDs). ({sup 3}H)Spiperone binding was saturable, high affinity and high density. Functionally, DA stimulated prostaglandin E{sub 2} production by IMCD cells, an effect that could be blocked by the DA{sub 2} antagonist domperidone. These results indicate that the kidney inner medulla expresses a functional DA receptor that may represent a newly identified DA receptor subtype (here designated DA{sub 2K}). Moreover, these results suggest that the kidney inner medulla may be a significant site at which DA, either directly or indirectly, influences water and electrolyte excretion.

• OSTI ID: 6095185
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:8; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550604* -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DIURETICS
DOPAMINE
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HYDROXY COMPOUNDS
INHIBITION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
KINETICS
MEMBRANE PROTEINS
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PROSTAGLANDINS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SPIPERONE
SYMPATHOMIMETICS