Effect of pentachlorophenol on the activation of 2,6-dinitrotoluene to genotoxic urinary metabolites in CD-1 mice: A comparison of G1 enzyme activities and urine mutagenicity
- US Environmental Protection Agency, Research Triangle Park, NC (United States)
- Environmental Health Research and Testing, Inc., Research Triangle Park, NC (United States)
2,6-Dinitrotoluene (2,6-DNT) and pentachlorophenol (PCP) are used for industrial purposes and are found in the environment as hazardous contaminants. Because concurrent exposure to both compounds can occur, it is of interest to determine if organochlorine compounds potentiate the effect of nitroaromatic chemicals. A significant increase in mutagenicity was observed in urines from mice treated with 2,6-DNT alone and in combination with PCP. By week 4, mice that received both 2,6-DNT and PCP excreted urine that was more mutagenic than that from animals which received only 2,6-DNT. At weeks 2 and 4, mice were sacrificed and intestinal enzyme activities (nitroreductase, azo reductase, {beta}-glucuronidase, dechlorinase, and dehydrochlorinase) were quantitated. The enhanced genotoxicity observed in urines from 2,6-DNT/PCP-treated mice coincided with a decrease in nitroreductase and an increase in {beta}-glucuronidase activities in the small intestine.
- OSTI ID:
- 6093868
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Vol. 18:2; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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CHLORINATED AROMATIC HYDROCARBONS
BIOLOGICAL EFFECTS
GASTROINTESTINAL TRACT
ENZYME ACTIVITY
POLYCYCLIC NITRO COMPOUNDS
CHEMICAL ACTIVATION
BIOASSAY
METABOLITES
PHENOLS
TOLUENE
URINE
ALKYLATED AROMATICS
AROMATICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
DIGESTIVE SYSTEM
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
HYDROXY COMPOUNDS
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ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
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560300* - Chemicals Metabolism & Toxicology