Effects of a calcium channel agonist on the electrical, ionic and secretory events in mouse pancreatic B-cells

Journal Article · Mon Sep 16 04:00:00 EDT 1985 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(85)91336-1· OSTI ID:6085407

Henquin, J C; Schmeer, W; Nenquin, M; Meissner, H P

The changes in pancreatic B-cell function produced by a Ca channel agonist, the dihydropyridine derivative CGP 28392, have been studied with mouse islets. CGP 28392 (5 microM) modified the electrical activity induced in B-cells by 10 mM glucose: the duration and the amplitude of the slow waves of membrane potential increased, but the overall spike activity decreased. Simultaneously, CGP 28392 markedly increased insulin release and UVCaS efflux, and slightly accelerated YWRb efflux from islet cells. These latter effects were abolished by omission of extracellular CaS . Qualitatively similar changes were observed at 15 mM glucose, whereas CGP 28392 was ineffective at 3 mM glucose. These results strongly suggest that an influx of CaS contributes to the slow waves of membrane potential triggered by glucose, and underline the importance of this influx of CaS for the control of insulin release by the sugar.

• Research Organization: Univ. of Saarland, Germany, F.R.
• OSTI ID: 6085407
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 2; ISSN BBRCA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOELECTRICITY
BIOLOGICAL EFFECTS
BODY
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARBOHYDRATES
CATIONS
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRICITY
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
GLUCOSE
HETEROCYCLIC COMPOUNDS
HEXOSES
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
IONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MEMBRANE TRANSPORT
MICE
MINUTES LIVING RADIOISOTOPES
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HORMONES
PYRIDINES
RADIOISOTOPES
RODENTS
RUBIDIUM 86
RUBIDIUM COMPOUNDS
RUBIDIUM ISOTOPES
SACCHARIDES
VERTEBRATES