Conversion of starch to ethanol in a recombinant saccharomyces cerevisiae strain expressing rice [alpha]-amylase from a novel Pichia pastoris alcohol oxidase promoter

Journal Article · Sat May 01 04:00:00 EDT 1993 · Bio/Technology; (United States)

DOI:https://doi.org/10.1038/nbt0593-606· OSTI ID:6084916

Kumagai, M H; Sverlow, G G; della-Cioppa, G; Grill, L K ^[1]

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1. Biosource Genetics Corporation, Vacaville, CA (United States)

A recombinant Saccharomyces cerevisiae, expressing and secreting rice [alpha]-amylase, converts starch to ethanol. The rice [alpha]-amylase gene (OS103) was placed under the transcriptional control of the promoter from a newly described Pichia pastoris alcohol oxidase genomic clone. The nucleotide sequences of ZZA1 and other methanol-regulated promoters were analyzed. A highly conserved sequence (TTG-N[sub 3]-GCTTCCAA-N[sub 5]-TGGT) was found in the 5' flanking regions of alcohol oxidase, methanol oxidase, and dihydroxyacetone synthase genes in Pichia pastoris, Hansenula polymorpha, and Candida biodinii S2. The yeast strain containing the ZZA1-OS103 fusion secreted biologically active enzyme into the culture media while fermenting soluble starch. 45 refs., 8 figs.

• OSTI ID: 6084916
• Journal Information: Bio/Technology; (United States), Journal Name: Bio/Technology; (United States) Vol. 11:5; ISSN 0733-222X; ISSN BTCHDA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
BIOSYNTHESIS
BIOTECHNOLOGY
DNA SEQUENCING
ETHANOL
EUMYCOTA
FUNGI
GENE RECOMBINATION
HYDROXY COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
YEASTS