Conversion of starch to ethanol in a recombinant saccharomyces cerevisiae strain expressing rice [alpha]-amylase from a novel Pichia pastoris alcohol oxidase promoter

Journal Article · Sat May 01 00:00:00 EDT 1993 · Bio/Technology; (United States)

DOI:https://doi.org/10.1038/nbt0593-606· OSTI ID:6084916

Kumagai, M H; Sverlow, G G; della-Cioppa, G; Grill, L K ^[1]

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1. Biosource Genetics Corporation, Vacaville, CA (United States)

A recombinant Saccharomyces cerevisiae, expressing and secreting rice [alpha]-amylase, converts starch to ethanol. The rice [alpha]-amylase gene (OS103) was placed under the transcriptional control of the promoter from a newly described Pichia pastoris alcohol oxidase genomic clone. The nucleotide sequences of ZZA1 and other methanol-regulated promoters were analyzed. A highly conserved sequence (TTG-N[sub 3]-GCTTCCAA-N[sub 5]-TGGT) was found in the 5' flanking regions of alcohol oxidase, methanol oxidase, and dihydroxyacetone synthase genes in Pichia pastoris, Hansenula polymorpha, and Candida biodinii S2. The yeast strain containing the ZZA1-OS103 fusion secreted biologically active enzyme into the culture media while fermenting soluble starch. 45 refs., 8 figs.

• OSTI ID: 6084916
• Journal Information: Bio/Technology; (United States), Vol. 11:5; ISSN 0733-222X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ETHANOL
BIOSYNTHESIS
SACCHAROMYCES CEREVISIAE
GENE RECOMBINATION
BIOTECHNOLOGY
DNA SEQUENCING
ALCOHOLS
EUMYCOTA
FUNGI
HYDROXY COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
SACCHAROMYCES
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
YEASTS
550400* - Genetics
550200 - Biochemistry