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Growth kinetics and yield study on Chlorella pyrenoidosa in chemically defined media

Conference · · Dev. Ind. Microbiol.; (United States)
OSTI ID:6082552
A Chlorella culture free from heterotrophic bacteria was obtained by eliminating the bacteria with successive use of antibiotics and agar plants. The purified Chlorella was cultured in chemically defined media. Under a photon flux (16.7 mw/cmS) similar to insolation, both heterotrophic and mixotrophic cultures were luxurious but the growth rates of autotrophic cultures were reduced substantially. The Chlorella culture grew most rapidly at 30 C in the absence of heterotrophic bacteria, and the highest specific growth rates were 1.43 x 10 h and 0.46 x 10 h for mixotrophic and autotrophic cultures, respectively. The highest photosynthetic efficiency over its growth period was 2.9% for autotrophic cultures. Elimination of heterotrophic bacteria from Chlorella cultures improved the algal growth rate as well as biomass yield significantly. A parasite of 0.1- m size was identified. The motile microorganism played an important role in the growth of the Chlorella and appeared to be common to green algae. 16 references, 2 tables.
Research Organization:
Standard Oil Co., Naperville, IL
OSTI ID:
6082552
Report Number(s):
CONF-8208164-
Conference Information:
Journal Name: Dev. Ind. Microbiol.; (United States) Journal Volume: 24
Country of Publication:
United States
Language:
English