Biodegradation of crystal violet by the white rot fungus phanerochaete chrysosporium
Biodegradation of crystal violet (N,N,N',N',N',N''- hexamethylpararosaniline) in ligninolytic (nitrogen-limited) cultures of the white rot fungus Phanerochaete chrysosporium was demonstrated by the disappearance of crystal violet and by the identification of three metabolites (N,N,N',N',N'' -pentamethylpararosaniline, N,N,N',N'' -tetramethylpararosaniline, and N,N',N'' -trimethylpararosaniline) formed by sequential N-demethylation of the parent compound. Metabolite formation also occurred when crystal violet was incubated with the extracellular fluid obtained from ligninolytic cultures of this fungus, provided that an H2O2-generating system was supplied. This, as well as the fact that a purified ligninase catalyzed N-demethylation of crystal violet, demonstrated that biodegradation of crystal violet by this fungus is dependent, at least in part, upon its lignin-degrading system. In addition to crystal violet, six other triphenylmethane dyes (pararosaniline, cresol red, bromphenol blue, ethyl violet, malachite green, and brilliant green) were shown to be degraded by the lignin-degrading system of this fungus.
- Research Organization:
- Utah State Univ., Logan, UT (United States)
- OSTI ID:
- 6081228
- Report Number(s):
- PB-93-191609/XAB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540220 -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AMINES
BIODEGRADATION
CARBOHYDRATES
CHEMICAL REACTIONS
CHROMATOGRAPHY
COUNTING TECHNIQUES
DECOMPOSITION
DEVELOPED COUNTRIES
DYES
ELEMENTS
EUMYCOTA
FUNGI
HIGH PRESSURE
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
LIGNIN
MEASURING INSTRUMENTS
METABOLISM
METABOLITES
METHYL VIOLET
NITROGEN
NONMETALS
NORTH AMERICA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
PHANEROCHAETE
PLANTS
POLYSACCHARIDES
SACCHARIDES
SEPARATION PROCESSES
SEQUENTIAL SCANNING
SPECTROMETERS
TRIPHENYLMETHANE DYES
USA