Genomic clones for human cholinesterase

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6076499

Kott, M; Venta, P J; Larsen, J; Lockridge, O; La Du, B

A human genomic library was prepared from peripheral white blood cells from a single donor by inserting an MboI partial digest into BamHI poly-linker sites of EMBL3. This library was screened using an oligolabeled human cholinesterase cDNA probe over 700 bp long. The latter probe was obtained from a human basal ganglia cDNA library. Of approximately 2 million clones screened with high stringency conditions several positive clones were identified; two have been plaque purified. One of these clones has been partially mapped using restriction enzymes known to cut within the coded region of the cDNA for human serum cholinesterase. Hybridization of the fragments and their sizes are as expected if the genomic clone is cholinesterase. Sequencing of the DNA fragments in M13 is in progress to verify the identify of the clone and the location of introns.

• Research Organization: Univ. of Michigan Medical School, Ann Arbor
• OSTI ID: 6076499
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BRAIN
CARBOXYLESTERASES
CENTRAL NERVOUS SYSTEM
CHOLINESTERASE
CLONING
DNA
DNA SEQUENCING
DNA-CLONING
ENZYMES
ESTERASES
HYDROLASES
LEUKOCYTES
MAMMALS
MAN
MATERIALS
NERVOUS SYSTEM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES