Identification of proteins important for tetracycline (TC) binding to ribosomes by single protein omission reconstitution (SPORE) experiments
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6074881
TC inhibits protein synthesis in E. coli by interfering with aminoacyl-tRNA binding to the ribosomal A site, and there is strong evidence that such inhibition results from TC binding to a high affinity site on the 30S subunit. The SPORE approach has been used to define those 30S proteins that are potentially important for high affinity TC binding, measured as the (/sup 3/H)-TC co-sedimenting with the reconstitution particle through a sucrose density gradient. Reverse phase-HPLC has been used both to prepare ribosomal proteins and to analyze the protein content of reconstituted particles. The results obtained so far show that protein S7, as well as some proteins linked to S7 in the 30S assembly map, are important for TC binding, whereas other ribosomal proteins are not. These results are in very good accord with their earlier photoaffinity labeling studies that strongly implicated S7 as forming part of the TC binding site. Interestingly, protein S18, which is photolabeled by TC to a high extent but in a non-site specific manner, appears to be unimportant for TC binding.
- Research Organization:
- Univ. of Pennsylvania, Philadelphia
- OSTI ID:
- 6074881
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
Similar Records
Single protein omission reconstitution studies of tetracycline binding to the 30S subunit of Escherichia coli ribosomes
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Effect of single base changes and the absence of modified bases in 16S RNA on the reconstitution and function of Escherichia coli 30S ribosomes
Journal Article
·
Tue Jun 05 00:00:00 EDT 1990
· Biochemistry; (United States)
·
OSTI ID:5393748
Incorporation of single dinitrophenyl-modified proteins in to the 30S subunit of Escherichia coli ribosomes by total reconstitution for localization by immune electron microscopy
Thesis/Dissertation
·
Sat Dec 31 23:00:00 EST 1988
·
OSTI ID:6159165
Effect of single base changes and the absence of modified bases in 16S RNA on the reconstitution and function of Escherichia coli 30S ribosomes
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6151282
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CELL CONSTITUENTS
CHROMATOGRAPHY
DRUGS
ESCHERICHIA COLI
INHIBITION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MEMBRANE PROTEINS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANOIDS
PROTEINS
REACTION KINETICS
RECEPTORS
RIBOSOMES
SEPARATION PROCESSES
SYNTHESIS
TETRACYCLINES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CELL CONSTITUENTS
CHROMATOGRAPHY
DRUGS
ESCHERICHIA COLI
INHIBITION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MEMBRANE PROTEINS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANOIDS
PROTEINS
REACTION KINETICS
RECEPTORS
RIBOSOMES
SEPARATION PROCESSES
SYNTHESIS
TETRACYCLINES
TRACER TECHNIQUES
TRITIUM COMPOUNDS