Keratins as markers that distinguish normal and tumor-derived mammary epithelial cells
- Dana-Farber Cancer Institute, Boston, MA (USA)
Keratin 5 (K5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived dell lines. To extend these findings, the full complements of keratins in normal, immortalized, and tumor cells were compared. It is shown here that normal cells produce keratins K5, K6, K7, K14, and K17, whereas tumor cells produce mainly keratins K8, K18, and K19. In immortalized cells, which are preneoplastic or partially transformed, the levels of K5 mRNA and protein are lower than in normal cells, whereas the amount of K18 is increased. Thus, K5 is an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decrease K5 expression correlates with tumorigenic progression.
- OSTI ID:
- 6073590
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:6; ISSN 0027-8424; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MARKERS
BODY
CARBOXYLIC ACIDS
CARCINOGENESIS
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
ELECTROPHORESIS
EPITHELIUM
EVEN-ODD NUCLEI
GLANDS
ISOTOPES
KERATIN
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMARY GLANDS
MESSENGER-RNA
METHIONINE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOGENESIS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RNA
SCLEROPROTEINS
SULFUR 35
SULFUR ISOTOPES
TISSUES
TUMOR CELLS