Endotoxin suppresses surfactant synthesis in cultured rat lung cells
Journal Article
·
· J. Trauma; (United States)
Pulmonary complications secondary to postburn sepsis are a major cause of death in burned patients. Using an in vitro organotypic culture system, we examined the effect of E. coli endotoxin (LPS) on lung cell surfactant synthesis. Our results showed that E. coli endotoxin (1.0, 2.5, 10 micrograms LPS/ml) was capable of suppressing the incorporation of /sup 3/H-choline into de novo synthesized surfactant, lamellar bodies (LB), and common myelin figures (CMF) at 50%, 68%, and 64%, respectively. In a similar study, we were able to show that LPS also inhibited /sup 3/H-palmitate incorporation by cultured lung cells. LPS-induced suppression of surfactant synthesis was reversed by hydrocortisone. Our results suggest that LPS may play a significant role in reducing surfactant synthesis by rat lung cells, and thus contribute to the pathogenesis of sepsis-related respiratory distress syndrome (RDS) in burn injury.
- Research Organization:
- Massachusetts General Hospital, Boston (USA)
- OSTI ID:
- 6073575
- Journal Information:
- J. Trauma; (United States), Journal Name: J. Trauma; (United States) Vol. 29:2; ISSN JOTRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ANTIGENS
BACTERIA
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
BURNS
CARBOXYLIC ACIDS
CELL CULTURES
CHOLINE
CORTICOSTEROIDS
DISEASES
DNA
DRUGS
ENDOTOXINS
ESCHERICHIA COLI
GLUCOCORTICOIDS
HEXADECANOIC ACID
HYDROCORTISONE
HYDROXY COMPOUNDS
INJURIES
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
LUNGS
MAMMALS
MATERIALS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PREGNANES
QUATERNARY COMPOUNDS
RATS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
STEROIDS
SURFACTANTS
SYNTHESIS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ANTIGENS
BACTERIA
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
BURNS
CARBOXYLIC ACIDS
CELL CULTURES
CHOLINE
CORTICOSTEROIDS
DISEASES
DNA
DRUGS
ENDOTOXINS
ESCHERICHIA COLI
GLUCOCORTICOIDS
HEXADECANOIC ACID
HYDROCORTISONE
HYDROXY COMPOUNDS
INJURIES
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
LUNGS
MAMMALS
MATERIALS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PREGNANES
QUATERNARY COMPOUNDS
RATS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
STEROIDS
SURFACTANTS
SYNTHESIS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES