Cytochrome P450-dependent arachidonic acid metabolites, 19- and 20-hydroxyeicosatetraenoic acids, enhance sodium-potassium ATPase activity in vascular smooth muscle

Journal Article · Sat Sep 01 04:00:00 EDT 1990 · Journal of Cardiovascular Pharmacology; (USA)

DOI:https://doi.org/10.1097/00005344-199009000-00013· OSTI ID:6073423

Escalante, B; Sessa, W C; Falck, J R; Yadagiri, P; Schwartzman, M L ^[1]

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1. New York Medical College, Valhalla (USA)

Several cytochrome P450-dependent arachidonic acid metabolites have been shown to affect Na+,K(+)-ATPase activity. In the present study, we tested the effect of omega- and omega - 1-hydroxylated products, i.e., 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE), on the K-induced relaxation in rat aortic rings. 19-HETE and 20-HETE increased the magnitude of the potassium-induced relaxation in a dose-dependent fashion (10(-7)-10(-5) M). The inhibitory effect of ouabain on the potassium-induced relaxation was reversed by both 19- and 20-HETE. In addition, indomethacin fully inhibited the stimulatory effect of 19- and 20-HETE on relaxation induced by potassium. Vascular ouabain-sensitive 86Rb uptake was also increased by 19- and 20-HETE. These observations suggest that 19- and 20-HETE stimulate vascular Na+,K(+)-ATPase via their conversion by cyclooxygenase to prostaglandin-like material.

• OSTI ID: 6073423
• Journal Information: Journal of Cardiovascular Pharmacology; (USA), Journal Name: Journal of Cardiovascular Pharmacology; (USA) Vol. 16:3; ISSN 0160-2446; ISSN JCPCD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALI METAL ISOTOPES
ALKALI METALS
ANIMALS
AORTA
ARACHIDONIC ACID
ARTERIES
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BLOOD VESSELS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CYTOCHROMES
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EICOSANOIC ACID
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GLYCOSIDES
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
METABOLITES
METALS
MINUTES LIVING RADIOISOTOPES
MONOCARBOXYLIC ACIDS
MUSCLES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OUABAIN
PHOSPHOHYDROLASES
PIGMENTS
POTASSIUM
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SODIUM
STROPHANTHINS
TRACER TECHNIQUES
VERTEBRATES