Radiation-induced association of beta-glucuronidase with purified nuclei from irradiated MOLT-4 and HeLa cells

Journal Article · Wed Feb 01 00:00:00 EST 1989 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577323· OSTI ID:6073032

McClain, D E; Kalinich, J F; Poplack, J K; Snyder, S L

Beta-glucuronidase, a lysosomal marker enzyme, associates with purified nuclei from HeLa and MOLT-4 cell lines in a radiation dose-dependent manner, up to 300 cGy in MOLT-4 cells, and 1000 cGy in HeLa cells. In MOLT-4 cells (200-cGy exposure), there is a significant increase in beta-glucuronidase activity detected in the nuclear fraction 24 h postirradiation with a maximum association occurring at 72 h. In HeLa cells (1000-cGy exposure), a significant association is first detected 24 h postirradiation with a maximum association at 48 h. The association is not the result of nonspecific contamination occurring during nuclei purification since nuclei from irradiated cells show no greater levels of plasma membrane marker and mitochondrial marker than controls. The nature of the association remains unclear, but activity is not removed by detergents used in the nuclei isolation procedure, and incubation of the nuclei with EDTA reverses the association only modestly. Exposure of nuclei from irradiated cells to anisotonic buffers also results in only a small decrease in beta-glucuronidase activity associated with the nuclei. These observations suggest that lysosomal hydrolases become intimately associated with the nuclei of irradiated cells.

• Research Organization: Armed Forces Radiobiology Research Institute, Bethesda, MD (USA)
• OSTI ID: 6073032
• Journal Information: Radiat. Res.; (United States), Vol. 117:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GLUCURONIDASE
ENZYME ACTIVITY
HELA CELLS
BIOLOGICAL RADIATION EFFECTS
TUMOR CELLS
CELL CULTURES
CELL NUCLEI
DOSE-RESPONSE RELATIONSHIPS
EDTA
LYSOSOMES
SURVIVAL TIME
AMINO ACIDS
ANIMAL CELLS
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHELATING AGENTS
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
RADIATION EFFECTS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture