Automatic analysis of flow cytometric DNA histograms from irradiated mouse male germ cells
An automatic procedure for recovering the DNA content distribution of mouse irradiated testis cells from flow cytometric histograms is presented. First, a suitable mathematical model is developed, to represent the pattern of DNA content and fluorescence distribution in the sample. Then a parameter estimation procedure, based on the maximum likelihood approach, is constructed by means of an optimization technique. This procedure has been applied to a set of DNA histograms relative to different doses of 0.4-MeV neutrons and to different time intervals after irradiation. In each case, a good agreement between the measured histograms and the corresponding fits has been obtained. The results indicate that the proposed method for the quantitative analysis of germ cell DNA histograms can be usefully applied to the study of the cytotoxic and mutagenic action of agents of toxicological interest such as ionizing radiations.18 references.
- Research Organization:
- Istituto di Analisi dei Sistemi ed Informatica del CNR, Rome (Italy)
- OSTI ID:
- 6073001
- Journal Information:
- Cytometry (Baltimore); (United States), Vol. 10:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
QUANTITATIVE CHEMICAL ANALYSIS
SPERMATOZOA
GENETIC RADIATION EFFECTS
CELL FLOW SYSTEMS
FLUORESCENCE
IONIZING RADIATIONS
MATHEMATICAL MODELS
MICE
NEUTRONS
REVIEWS
TESTES
ANIMALS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CHEMICAL ANALYSIS
DOCUMENT TYPES
ELEMENTARY PARTICLES
FERMIONS
GAMETES
GENETIC EFFECTS
GERM CELLS
GONADS
HADRONS
LUMINESCENCE
MALE GENITALS
MAMMALS
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture