Mutagenicity of the fractionated organic emissions from diesel, cigarette smoke condensate, coke oven, and roofing tar in the Ames assay

Technical Report · Tue Jan 01 04:00:00 EST 1985

OSTI ID:6072987

Austin, A C; Claxton, L D; Lewtas, J

Mobile and stationary sources emit particle-bound organics that have demonstrated mutagenicity. The objective of this study was to measure the mutagenicity of the fractionated organic emissions from diesel, cigarette smoke condensate (CSC), coke ovens and roofing tar in the Ames assay. This study demonstrated significant biological differences among the four emission sources. Within each source, the relative mutagenicity of each fraction was significantly different in the presence and absence of an exogenous metabolic activation. In the diesel sample, over 90% of the mutagenic activity is located in the aromatic and polar neutral (PN) fractions, and a significant portion of this activity can be accounted for by nitro-PNAs. Most of the mutagenicity of the coke-oven main sample was found in the BASE and PN fractions which contained aromatic amines and nitrogen heterocycles. The CSC sample also had a high percent of the mutagenic activity in the BASE fraction. Chemical analysis however, indicates that the components in the CSC differed significantly from those of the coke-oven main sample. The roofing-tar sample contained aromatic and polar mutagenic constituents that were not NO/sub 2/-PNAs. Although the specific mutagens in these different sources are not identical, they all cause frameshift mutations and appear to be compounds that could be classified as polycyclic organic matter (POM).

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
• OSTI ID: 6072987
• Report Number(s): PB-86-118809/XAB
• Country of Publication: United States
• Language: English

Related Subjects

500200* -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
AEROSOLS
AIR POLLUTION
AROMATICS
BIOASSAY
CARBONIZATION
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
COKE OVENS
COKING
COLLOIDS
COMBUSTION PRODUCTS
DECOMPOSITION
DIESEL FUELS
DISPERSIONS
EMISSION
HYDROCARBONS
INDUSTRIAL WASTES
MUTAGENS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PETROLEUM PRODUCTS
POLLUTION
POLYCYCLIC AROMATIC HYDROCARBONS
RESIDUES
SMOKES
SOLS
TAR
TOXICITY
WASTES