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Title: Photolabeling of the alpha-neuraminidase/beta-galactosidase complex from human placenta with a photoreactive neuraminidase inhibitor

Abstract

Photolabeling of the alpha-neuraminidase/beta-galactosidase complex in human placenta was carried out using the radioactive photoprobe, 9-S-(4-azido-3,5-3H-2-nitrophenyl)-5-acetamido-2,6 anhydro-2,3,5,9- tetradeoxy-9- thio-D-glycero-D-galacto-non-2-enonic acid. Two intensely labeled bands at 61 and 46 kD were detected with autoradiography. Labeling of the 46 kD protein was blocked with the inclusion of the surfactant Triton X-100 in the photolysis mixture, indicating a nonspecific, hydrophobic interaction. The 61 kD protein was protected from labeling only when the neuraminidase inhibitor 2,3 dehydro N-acetyl neuraminic acid (1 mM) was present during photolysis. These results suggest that the neuraminidase activity resides among the proteins in the 61 kD molecular weight range comigrating with the lysosomal beta-galactosidase, under denaturing conditions.

Authors:
; ;  [1]
  1. (Univ. of Tennessee, Memphis (USA))
Publication Date:
OSTI Identifier:
6072439
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; (USA); Journal Volume: 173:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GALACTOSIDASE; LABELLING; GLYCOSYL HYDROLASES; AFFINITY; AUTORADIOGRAPHY; COMPLEXES; ELECTROPHORESIS; ENZYME ACTIVITY; ENZYME INHIBITORS; MAN; MOLECULAR WEIGHT; PLACENTA; TRITIUM COMPOUNDS; ANIMALS; ENZYMES; FETAL MEMBRANES; HYDROGEN COMPOUNDS; HYDROLASES; MAMMALS; MEMBRANES; O-GLYCOSYL HYDROLASES; PRIMATES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Warner, T.G., Louie, A., and Potier, M. Photolabeling of the alpha-neuraminidase/beta-galactosidase complex from human placenta with a photoreactive neuraminidase inhibitor. United States: N. p., 1990. Web. doi:10.1016/S0006-291X(05)81014-9.
Warner, T.G., Louie, A., & Potier, M. Photolabeling of the alpha-neuraminidase/beta-galactosidase complex from human placenta with a photoreactive neuraminidase inhibitor. United States. doi:10.1016/S0006-291X(05)81014-9.
Warner, T.G., Louie, A., and Potier, M. Fri . "Photolabeling of the alpha-neuraminidase/beta-galactosidase complex from human placenta with a photoreactive neuraminidase inhibitor". United States. doi:10.1016/S0006-291X(05)81014-9.
@article{osti_6072439,
title = {Photolabeling of the alpha-neuraminidase/beta-galactosidase complex from human placenta with a photoreactive neuraminidase inhibitor},
author = {Warner, T.G. and Louie, A. and Potier, M.},
abstractNote = {Photolabeling of the alpha-neuraminidase/beta-galactosidase complex in human placenta was carried out using the radioactive photoprobe, 9-S-(4-azido-3,5-3H-2-nitrophenyl)-5-acetamido-2,6 anhydro-2,3,5,9- tetradeoxy-9- thio-D-glycero-D-galacto-non-2-enonic acid. Two intensely labeled bands at 61 and 46 kD were detected with autoradiography. Labeling of the 46 kD protein was blocked with the inclusion of the surfactant Triton X-100 in the photolysis mixture, indicating a nonspecific, hydrophobic interaction. The 61 kD protein was protected from labeling only when the neuraminidase inhibitor 2,3 dehydro N-acetyl neuraminic acid (1 mM) was present during photolysis. These results suggest that the neuraminidase activity resides among the proteins in the 61 kD molecular weight range comigrating with the lysosomal beta-galactosidase, under denaturing conditions.},
doi = {10.1016/S0006-291X(05)81014-9},
journal = {Biochemical and Biophysical Research Communications; (USA)},
number = ,
volume = 173:1,
place = {United States},
year = {Fri Nov 30 00:00:00 EST 1990},
month = {Fri Nov 30 00:00:00 EST 1990}
}