Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Journal of Clinical Microbiology; (USA)

OSTI ID:6070130

Collins, M T; Kenefick, K B; Sockett, D C; Lambrecht, R S; McDonald, J; Jorgensen, J B ^[1]

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1. Univ. of Wisconsin, Madison (USA)

A commercial radiometric medium, BACTEC 12B, was modified by addition of mycobactin, egg yolk suspension, and antibiotics (vancomycin, amphotericin B, and nalidixic acid). Decontaminated bovine fecal specimens were filter concentrated by using 3-microns-pore-size, 13-mm-diameter polycarbonate filters, and the entire filter was placed into the radiometric broth. Comparison of the radiometric technique with conventional methods on 603 cattle from 9 Mycobacterium paratuberculosis-infected herds found that of 75 positive specimens, the radiometric technique detected 92% while conventional methods detected 60% (P less than 0.0005). Only 3.9% of radiometric cultures were contaminated. To measure the effect of filter concentration of specimens on the detection rate, 5 cattle with minimal and 5 with moderate ileum histopathology were sampled weekly for 3 weeks. M. paratuberculosis was detected in 33.3% of nonfiltered specimens and 76.7% of filtered specimens (P less than 0.005). Detection rates were directly correlated with the severity of disease, and the advantage of specimen concentration was greatest on fecal specimens from cattle with low-grade infections. Detection times were also correlated with infection severity: 13.4 +/- 5.9 days with smear-positive specimens, 27.9 +/- 8.7 days with feces from cows with typical subclinical infections, and 38.7 +/- 3.8 days with fecal specimens from cows with low-grade infections. Use of a cocktail of vancomycin, amphotericin B, and nalidixic acid for selective suppression of nonmycobacterial contaminants was better than the commercial product PANTA (Becton Dickinson Microbiologic Systems, Towson, Md.) only when specimens contained very low numbers of M. paratuberculosis.

• OSTI ID: 6070130
• Journal Information: Journal of Clinical Microbiology; (USA), Vol. 28:11; ISSN 0095-1137
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
FECES
RADIOMETRIC ANALYSIS
TUBERCULOSIS
DIAGNOSIS
CATTLE
CULTURE MEDIA
FILTRATION
MYCOBACTERIUM TUBERCULOSIS
SENSITIVITY ANALYSIS
ANIMALS
BACTERIA
BACTERIAL DISEASES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
CHEMICAL ANALYSIS
DISEASES
DOMESTIC ANIMALS
INFECTIOUS DISEASES
MAMMALS
MATERIALS
MICROORGANISMS
MYCOBACTERIUM
QUANTITATIVE CHEMICAL ANALYSIS
RUMINANTS
SEPARATION PROCESSES
VERTEBRATES
WASTES
550601* - Medicine- Unsealed Radionuclides in Diagnostics