Animal model of pulmonary radiation fibrosis with biochemical, physiologic, immunologic, and morphologic observations

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577022· OSTI ID:6069490

Karvonen, R L; Fernandez-Madrid, F; Maughan, R L; Palmer, K C; Fernandez-Madrid, I

An animal model of pulmonary radiation fibrosis was established, using male CBA/j mice. Both lungs of each mouse in one group (DL) were irradiated with two doses of 8.5 Gy each, separated by 30 days. A control group (CG) was sham-irradiated. There was a small but significant difference in average breathing rate between DL and CG 27 weeks after the second irradiation which increased until the 34th week followed by a plateau. The accumulated hydroxyproline content of the irradiated mouse lung was 40% greater than that of the sham-irradiated lung at 42 weeks and thereafter. Anticollagen antibodies assayed 52 weeks after irradiation by enzyme-linked immunosorbent assay were elevated by 49% in sera from the irradiated mice compared to sera from sham-irradiated mice. Mortality during the 52-week period following the second irradiation was low (13%) for both groups. Histological comparison of irradiated and control mouse lungs fixed under uniform inflation pressure indicated no significant differences. The model has unique features including an increase in collagen deposition, no acute changes attributable to radiation, a small but statistically significant abnormality in pulmonary function, an immunologic response to collagen, and low mortality.

• Research Organization: Wayne State Univ. School of Medicine, Detroit, MI
• OSTI ID: 6069490
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 111:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
BODY
CHEMISTRY
COLLAGEN
FIBROSIS
FUNCTIONS
IMMUNE REACTIONS
IMMUNOASSAY
INJURIES
LUNGS
MAMMALS
MEDICINE
MICE
MORPHOLOGY
MORTALITY
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PROTEINS
RADIATION EFFECTS
RADIATION INJURIES
RADIOLOGY
RADIOTHERAPY
RESPIRATION
RESPIRATORY SYSTEM
RODENTS
SCLEROPROTEINS
THERAPY
VERTEBRATES