Semiquantitative measure of immune responses against erythropoietic stem cell antigens
Journal Article
·
· Immunogenetics (N.Y.); (United States)
A semiquantitative assay was developed and used to measure the effects of immune responses against 16 independent non-H-2 antigenic loci on erythropoietic stem cells. The assay compares repopulation in genetically anemic WBB6F1-W/Wv recipients that have normal immune responses, and in lethally irradiated WBB6F1 +/+ mice whose immune responses are suppressed by the irradiation. The differences in repopulating ability between these two types of recipients measure how immune responses affect erythropoietic stem cells. Stem cell repopulating abilities for the cells with antigens specified by the Thy-1, H-1, H-24, Ly-1, H-37, and H-17 loci were affected slightly, if at all. Repopulating abilities were moderately reduced by responses against antigens specified by H-15, 16, Ea-2, and Ly-2, 3 loci, and against the differences between the B6 and B10 genotypes, although marrow of these types cured W/Wv recipients. A surprising result occurred for the antigen specified by the H-8 locus, in which immune responses strongly reduced repopulating abilities, although this type of marrow cell cured W/Wv recipients. A comparison of these results with skin graft survival times suggests that the antigens specified by the H-17 and H-24 loci are strongly immunogenic on skin but not on marrow stem cells, while those specified by the H-12 and H-8 loci are strongly immunogenic on marrow stem cells but not on skin.
- Research Organization:
- Jackson Laboratory, Bar Harbor, ME
- OSTI ID:
- 6068360
- Journal Information:
- Immunogenetics (N.Y.); (United States), Journal Name: Immunogenetics (N.Y.); (United States) Vol. 26:3; ISSN IMNGB
- Country of Publication:
- United States
- Language:
- English
Similar Records
Ultimate erythropoietic repopulating abilities of fetal, young adult, and old adult cells compared using repeated irradiation
Skin graft rejection in mice repopulated with marrow of the skin donor type: a Skn gene in a congenic line
Long-term erythropoietic repopulating ability of old, young, and fetal stem cells
Journal Article
·
Sat Sep 01 00:00:00 EDT 1984
· J. Exp. Med.; (United States)
·
OSTI ID:6051291
Skin graft rejection in mice repopulated with marrow of the skin donor type: a Skn gene in a congenic line
Journal Article
·
Sat Dec 31 23:00:00 EST 1983
· Immunogenetics (N.Y.); (United States)
·
OSTI ID:6220782
Long-term erythropoietic repopulating ability of old, young, and fetal stem cells
Journal Article
·
Sun May 01 00:00:00 EDT 1983
· J. Exp. Med.; (United States)
·
OSTI ID:5585711
Related Subjects
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGENS
BLOOD FORMATION
BODY
BONE MARROW
CHIMERAS
ERYTHROPOIESIS
GRAFTS
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
IRRADIATION
LETHAL IRRADIATION
MAMMALS
MICE
MOSAICISM
ORGANS
RADIATION CHIMERAS
RODENTS
SKIN
SOMATIC CELLS
STEM CELLS
SURVIVAL CURVES
TISSUES
TRANSPLANTS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGENS
BLOOD FORMATION
BODY
BONE MARROW
CHIMERAS
ERYTHROPOIESIS
GRAFTS
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
IRRADIATION
LETHAL IRRADIATION
MAMMALS
MICE
MOSAICISM
ORGANS
RADIATION CHIMERAS
RODENTS
SKIN
SOMATIC CELLS
STEM CELLS
SURVIVAL CURVES
TISSUES
TRANSPLANTS
VERTEBRATES