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Title: Semiquantitative measure of immune responses against erythropoietic stem cell antigens

Abstract

A semiquantitative assay was developed and used to measure the effects of immune responses against 16 independent non-H-2 antigenic loci on erythropoietic stem cells. The assay compares repopulation in genetically anemic WBB6F1-W/Wv recipients that have normal immune responses, and in lethally irradiated WBB6F1 +/+ mice whose immune responses are suppressed by the irradiation. The differences in repopulating ability between these two types of recipients measure how immune responses affect erythropoietic stem cells. Stem cell repopulating abilities for the cells with antigens specified by the Thy-1, H-1, H-24, Ly-1, H-37, and H-17 loci were affected slightly, if at all. Repopulating abilities were moderately reduced by responses against antigens specified by H-15, 16, Ea-2, and Ly-2, 3 loci, and against the differences between the B6 and B10 genotypes, although marrow of these types cured W/Wv recipients. A surprising result occurred for the antigen specified by the H-8 locus, in which immune responses strongly reduced repopulating abilities, although this type of marrow cell cured W/Wv recipients. A comparison of these results with skin graft survival times suggests that the antigens specified by the H-17 and H-24 loci are strongly immunogenic on skin but not on marrow stem cells, while those specified by themore » H-12 and H-8 loci are strongly immunogenic on marrow stem cells but not on skin.« less

Authors:
Publication Date:
Research Org.:
Jackson Laboratory, Bar Harbor, ME
OSTI Identifier:
6068360
Resource Type:
Journal Article
Resource Relation:
Journal Name: Immunogenetics (N.Y.); (United States); Journal Volume: 26:3
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; SKIN; GRAFTS; STEM CELLS; IMMUNE REACTIONS; ANTIGENS; BONE MARROW; ERYTHROPOIESIS; LETHAL IRRADIATION; MICE; RADIATION CHIMERAS; SURVIVAL CURVES; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BLOOD FORMATION; BODY; CHIMERAS; HEMATOPOIETIC SYSTEM; IRRADIATION; MAMMALS; MOSAICISM; ORGANS; RODENTS; SOMATIC CELLS; TISSUES; TRANSPLANTS; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals

Citation Formats

Harrison, D.E. Semiquantitative measure of immune responses against erythropoietic stem cell antigens. United States: N. p., 1987. Web. doi:10.1007/BF00365900.
Harrison, D.E. Semiquantitative measure of immune responses against erythropoietic stem cell antigens. United States. doi:10.1007/BF00365900.
Harrison, D.E. Thu . "Semiquantitative measure of immune responses against erythropoietic stem cell antigens". United States. doi:10.1007/BF00365900.
@article{osti_6068360,
title = {Semiquantitative measure of immune responses against erythropoietic stem cell antigens},
author = {Harrison, D.E.},
abstractNote = {A semiquantitative assay was developed and used to measure the effects of immune responses against 16 independent non-H-2 antigenic loci on erythropoietic stem cells. The assay compares repopulation in genetically anemic WBB6F1-W/Wv recipients that have normal immune responses, and in lethally irradiated WBB6F1 +/+ mice whose immune responses are suppressed by the irradiation. The differences in repopulating ability between these two types of recipients measure how immune responses affect erythropoietic stem cells. Stem cell repopulating abilities for the cells with antigens specified by the Thy-1, H-1, H-24, Ly-1, H-37, and H-17 loci were affected slightly, if at all. Repopulating abilities were moderately reduced by responses against antigens specified by H-15, 16, Ea-2, and Ly-2, 3 loci, and against the differences between the B6 and B10 genotypes, although marrow of these types cured W/Wv recipients. A surprising result occurred for the antigen specified by the H-8 locus, in which immune responses strongly reduced repopulating abilities, although this type of marrow cell cured W/Wv recipients. A comparison of these results with skin graft survival times suggests that the antigens specified by the H-17 and H-24 loci are strongly immunogenic on skin but not on marrow stem cells, while those specified by the H-12 and H-8 loci are strongly immunogenic on marrow stem cells but not on skin.},
doi = {10.1007/BF00365900},
journal = {Immunogenetics (N.Y.); (United States)},
number = ,
volume = 26:3,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1987},
month = {Thu Jan 01 00:00:00 EST 1987}
}
  • This study establishes assay systems for helper T cell activities assisting cytotoxic T lymphocyte (CTL) and antibody responses to tumor-associated antigens (TAA) and demonstrates the existence of TAA that induce preferentially anti-TAA CTL helper and B cell helper T cell activities in two syngeneic tumor models. C3H/HeN mice were immunized to the syngeneic X5563 plasmacytoma or MH135 hepatoma. Spleen cells from these mice were tested for anti-TAA helper T cell activity capable of augmenting anti-trinitrophenyl(TNP) CTL and anti-TNP antibody responses from anti-TNP CTL and B cell precursors (responding cells) by stimulation with TNP-modified X5563 or MH134 tumor cells. The resultsmore » demonstrate that cultures of responding cells plus 850R X-irradiated tumor-immunized spleen cells (helper cells) failed to enhance anti-TNP CTL or antibody responses when in vitro stimulation was provided by either unmodified tumor cells or TNP-modified syngeneic spleen cells (TNP-self). In contrast, these cultures resulted in appreciable augmentation of anti-TNP CTL or antibody response when stimulated by TNP-modified tumor cells. The results are discussed in the light of cellular mechanisms underlying the preferential anti-TAA immune responses, and the interrelationship between various types of cell functions including CTL- and B cell-help.« less
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