Sperm in poor quality semen from bulls during heat stress have a lower affinity for binding hydrogen-3 heparin

Journal Article · Thu Jan 01 04:00:00 EST 1987 · J. Dairy Sci.; (United States)

DOI:https://doi.org/10.3168/jds.S0022-0302(87)79994-9· OSTI ID:6065284

Ax, R L; Gilbert, G R; Shook, G E

Binding assays with (/sup 3/H) heparin were performed using spermatozoa collected prior to, during, and following summer heat stress to dairy bulls. Ejaculates collected in August 1983 after a period of ambient temperatures exceeding 29.4/sup 0/C exhibited a high frequency of abnormal sperm, and motility was reduced in some samples. Sperm in samples collected during heat stress possessed dissociation constants for binding (/sup 3/H) heparin ranging from 134.5 to 163.2 nmol. In contrast, sperm in semen collected prior to and after heat stress had significantly lower dissociation constants (higher affinity) for (/sup 3/H)heparin, 12.9 to 56.4 nmol. The number of binding sites for (/sup 3/H) heparin on sperm did not change among collection periods. It was concluded that the binding affinity for (/sup 3/H) heparin may reflect membrane integrity of bull sperm.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 6065284
• Journal Information: J. Dairy Sci.; (United States), Journal Name: J. Dairy Sci.; (United States) Vol. 70:1; ISSN JDSCA
• Country of Publication: United States
• Language: English

Related Subjects

553005* -- Agriculture & Food Technology-- Animal Husbandry-- (1987-)
60 APPLIED LIFE SCIENCES
AMINES
ANIMALS
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL STRESS
CARBOHYDRATES
CATTLE
DOMESTIC ANIMALS
DRUGS
FERTILITY
GAMETES
GERM CELLS
HEMATOLOGIC AGENTS
HEPARIN
HYDROGEN ISOTOPES
INJURIES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANES
MUCOPOLYSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
RADIOISOTOPES
REPRODUCTION
RUMINANTS
SACCHARIDES
SPERMATOZOA
TRACER TECHNIQUES
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES