Genetic studies on a nitrogen-fixing cyanobacterium. [Anabaena; Escherichi coli]
Mutants of Anabaena PCC7120 capable of aerobic growth with NO/sub 3//sup -/ but not N/sub 2/, and capable of microaerobic reduction of C/sub 2/H/sub 2/, were isolated by penicillin enrichment after UV irradiation. Heterocysts of two mutants lack the principal envelope glycolipid, those of EF116 have a non-cohesive envelope polysaccharide, and those of other strains have other defects. A Nm/sup r/ cosmid library of DNA from wild type Anabaena PCC7120 was established in Escherichia coli bearing the Ap helper plasmid pDS4101. A conjugative plasmid was introduced, and the bacteria replicated to lawns of individual mutant strains of Anabaena. After one day of non-selective growth, selection was applied for Nm/sup r/ and nitrogen fixation. Overlapping cosmids complementing EF116 and one complementing another mutant have been mapped. The complementing genes are thought to act early in differentiation. Inclusion, in an E. coli donor of an appropriate methylase gene enhanced, by a factor of 10/sup 2/ to 10/sup 3/, transfer to Anabaena PCC7120 of a plasmid containing numerous sites for the Anabaena restriction endonuclease, AvaII.
- Research Organization:
- Michigan State Univ., East Lansing
- OSTI ID:
- 6064448
- Report Number(s):
- CONF-8707108-
- Journal Information:
- Plant Physiol., Suppl.; (United States), Journal Name: Plant Physiol., Suppl.; (United States) Vol. 83:4; ISSN PPYSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560140* -- Radiation Effects on Plants
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROBIC CONDITIONS
ALKENES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
DNA-ASE
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
ENDONUCLEASES
ENZYMES
ESCHERICHIA COLI
ESTERASES
ETHYLENE
GENETIC EFFECTS
GENETIC ENGINEERING
GENETIC MAPPING
GENETIC RADIATION EFFECTS
HYDROCARBONS
HYDROLASES
MAPPING
MICROORGANISMS
MUTANTS
NITRATES
NITROGEN
NITROGEN COMPOUNDS
NITROGEN FIXATION
NONMETALS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PENICILLIN
PHOSPHODIESTERASES
PHOTOSYNTHETIC BACTERIA
PLASMIDS
RADIATION EFFECTS
RADIATIONS
ULTRAVIOLET RADIATION