Molybdenum site of xanthine oxidase. Structural evidence from x-ray absorption spectroscopy

Journal Article · Wed May 09 04:00:00 EDT 1979 · J. Am. Chem. Soc.; (United States)

DOI:https://doi.org/10.1021/ja00504a069· OSTI ID:6063384

Tullius, T D; Kurtz, Jr, D M; Conradson, S D; Hodgson, K O

Xanthine oxidase was isolated from unpasteurized buttermilk by a standard procedure. Extended x-ray absorption fine structure (EXAFS) data indicate that reduced xanthine oxidase contains molybdenum in the four valence oxidation state. Curve fitting of the EXAFS data for oxidized xanthine oxidase suggests that there are two different Mo-ligand distances, 1.5 oxygen atoms at 1.71 A and 2 sulfur atoms at 2.54 A. Including a third sulfur atom at a longer distance significantly improved the fit. Several possible formulations are proposed. 2 figures, 1 table.

• Research Organization: Stanford Univ., CA
• OSTI ID: 6063384
• Journal Information: J. Am. Chem. Soc.; (United States), Journal Name: J. Am. Chem. Soc.; (United States) Vol. 101:10; ISSN JACSA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201* -- Chemical & Physicochemical Properties
400301 -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
400400 -- Electrochemistry
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTRA
BOND LENGTHS
CHEMICAL REACTIONS
COMPLEXES
DATA
DATA FORMS
ENZYMES
EXPERIMENTAL DATA
HETEROCYCLIC COMPOUNDS
INFORMATION
ISOLATED VALUES
LIGANDS
MOLYBDENUM COMPLEXES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOMETALLIC COMPOUNDS
OXIDASES
OXIDATION
OXIDOREDUCTASES
PURINES
REDUCTION
SPECTRA
SPECTROSCOPY
STRUCTURAL CHEMICAL ANALYSIS
TRANSITION ELEMENT COMPLEXES
VALENCE
X-RAY SPECTROSCOPY
XANTHINES