mRNA decapping enzyme from ribosomes of Saccharomyces cerevisiae
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
By use of (/sup 3/H)methyl-5'-capped (/sup 14/C)mRNA from yeast as a substrate, a decapping enzyme activity has been detected in enzyme fractions derived from a high salt wash of ribosomes of Saccharomyces cerevisiae. The product of the decapping reaction is (/sup 3/H)m/sup 7/GDP. That the enzyme is not a non-specific pyrophosphatase is suggested by the finding that the diphosphate product, m/sup 7/GpppA(G), and UDP-glucose are not hydrolyzed.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6063244
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 96:3; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550401* -- Genetics-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CHEMISTRY
ENZYME ACTIVITY
ENZYMES
FUNGI
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MESSENGER-RNA
METABOLITES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PLANTS
REACTION KINETICS
RIBOSOMES
RNA
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
YEASTS
550401* -- Genetics-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CHEMISTRY
ENZYME ACTIVITY
ENZYMES
FUNGI
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MESSENGER-RNA
METABOLITES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PLANTS
REACTION KINETICS
RIBOSOMES
RNA
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
YEASTS