Induction of stress genes by heat-shock in teleost erythrocytes

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6062614

Koban, M; Yup, A A; Powers, D A

The eurythermal minnow Fundulus heteroclitus can tolerate rapid temperature changes of more than 20 C. Since heat-shock protein (hsp) synthesis and accumulation confers the transient thermotolerance in many cell types, they examined the heat-shock response of Fundulus for features that may correlate with its adaptability to thermal stress. Erythrocytes, prepared from 20 C acclimated fish, were heated at 40 c. Metabolically labeled (/sup 35/S-methionine) proteins were resolved by 2-d O'Farrell gels. Fundulus synthesize two major hsps of 74 kDa and 76 kDa respectively; each hsp is composed of 3 acidic polypeptides. The pattern of hsp synthesis showed interesting individual variability. Cells of some fish preferentially synthesized either hsp 76 or hsp 74; others synthesized both. While western immunoblots and silver-stained gels indicated that hsps are abundant proteins in unstressed cells, high rates of synthesis were only apparent during heat stress. According to RNA dot-blot analysis, heat-shock mRNA is present in unstressed cells but only increases by 50% following heat stress. Heat-shock RNA of unstressed cells is not translationally active because actinomycin-D inhibits synthesis of hsps. Hsps were synthesized within 15 min of heat stress and continued to be synthesized for more than 6 hours following recovery at 20 C.

• Research Organization: Johns Hopkins Univ., Baltimore, MD
• OSTI ID: 6062614
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550401 -- Genetics-- Tracer Techniques
560200* -- Thermal Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL ADAPTATION
BIOLOGICAL MATERIALS
BIOLOGICAL STRESS
BIOLOGICAL VARIABILITY
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ENERGY
ERYTHROCYTES
EVEN-ODD NUCLEI
FISHES
GENE REGULATION
GENES
GENETIC VARIABILITY
HEAT
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MATERIALS
METHIONINE
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
RNA
SENSITIVITY
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TEMPERATURE EFFECTS
TRACER TECHNIQUES
VERTEBRATES