Acetate catabolism by Methanosarcina barkeri
Cell suspensions of Methanosarcina barkeri convert the carboxyl and methyl group carbons of acetate to carbon dioxide and methane at pH 6 under an atmosphere of 100% CO/sub 2/. The rate of loss of radioactivity from (1-/sup 14/C)acetate was over three times greater than that from (2-/sup 14/C)acetate under these conditions. Control experiments with both labeled substrates present showed that the rates were additive. Addition of a high level of 2-bromoethanesulfonate to selectively inhibit methane formation largely inhibited release of /sup 14/C from methyl-labeled acetate but only marginally decreased the rate of loss from (1-/sup 14/C)acetate. Thus, in the absence of the inhibitor loss of /sup 14/C from (1-/sup 14/C)acetate likely reflects an isotopic exchange reaction with CO/sub 2/ superimposed on the overall conversion of acetate to CO/sub 2/ and CH/sub 4/. The exchange reaction was inhibited by uncouplers such as 2,4-dinitrophenol, CCCP, and FCCP. Cells permeabilized by treatment with nonionic detergents or disrupted by passage through a French pressure cell failed to catalyze the exchange reaction. Exchange activity was not restored by addition of ATP or by use of (1-/sup 14/C)acetyl CoA as substrate. No evidence for involvement of carbon monoxide dehydrogenase in the exchange was found in these experiments when CO/sub 2/ was replaced by CO. However, the soluble extracts retained the ability to convert acetate to methane in the presence of H/sub 2/ and ATP.
- Research Organization:
- National Institute of Health, Bethesda, MD
- OSTI ID:
- 6062189
- Report Number(s):
- CONF-870644-; TRN: 87-036543
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ACETATES
CATABOLISM
METHANE
SYNTHESIS
NITROPHENOL
BIOLOGICAL EFFECTS
ATP
CARBON 14 COMPOUNDS
CARBON DIOXIDE
HYDRAZONES
HYDROGEN
ISOTOPIC EXCHANGE
METHANOGENIC BACTERIA
TRACER TECHNIQUES
ALKANES
AROMATICS
BACTERIA
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACID SALTS
CHALCOGENIDES
ELEMENTS
HYDROCARBONS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
MICROORGANISMS
NITRO COMPOUNDS
NONMETALS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHENOLS
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550501 - Metabolism- Tracer Techniques