Decreased chromate uptake in Pseudomonas fluorescens carrying a chromate resistance plasmid

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · J. Bacteriol.; (United States)

OSTI ID:6061860

Ohtake, H; Cervantes, C; Silver, S

CrO/sub 4//sup 2 -/ resistance in Pseudomonas fluorescens LB300(pLHB1) was related to reduced uptake of CrO/sub 4//sup 2 -/ relative to the plasmidless strain LB303. /sup 51/CrO/sub 4//sup 2 -/ was transported mainly via the SO/sub 4//sup 2 -/ active transport system; thus, cells grown with 0.15 mM cysteine, a repressor of the SO/sub 4//sup 2 -/ transport system, were much more resistant to CrO/sub 4//sup 2 -/ than those grown with 0.15 mM djenkolic acid, which derepressed the /sup 35/SO/sub 4//sup 2 -/ uptake system. Kinetics of /sup 51/CrO/sub 4//sup 2 -/ uptake by P. fluorescens with and without the plasmid showed that the V/sub max/ for /sup 51/CrO/sub 4//sup 2 -/ uptake with the resistant strain was 2.2 times less than the V/sub max/ for the sensitive strain, whereas the K/sub m/ remained constant.

• Research Organization: Washington Univ., St. Louis, MO
• OSTI ID: 6061860
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 169:8; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
560400 -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL VARIABILITY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHROMATES
CHROMIUM 51
CHROMIUM COMPOUNDS
CHROMIUM ISOTOPES
CYSTEINE
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GENETIC VARIABILITY
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PLASMIDS
PSEUDOMONAS
RADIOISOTOPES
SENSITIVITY
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
THIOLS
TOXICITY
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
UPTAKE