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Biological effects of short-term, high-concentration exposure to methyl isocyanate. IV. Influence on the oxygen binding properties of guinea pig blood

Journal Article · · Environ. Health Perspect.; (United States)
DOI:https://doi.org/10.1289/ehp.877235· OSTI ID:6061099
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Whole blood oxygen equilibrium curves (O/sub 2/ECs), blood buffer lines, and several hematologic properties were determined for adult guinea pigs exposed to 700 ppm methyl isocyanate (MIC) for 15 min. MIC inhalation effected a significant reduction of blood O/sub 2/ affinity; the half-saturation pressure (P/sub 50/) at 38/sup 0/C increased from the control (untreated) level of 22.8 +/- 0.1 mm Hg to values ranging from 28.5 to 43.7 mm Hg for experimental animals. MIC exposure had no apparent influence on O/sub 2/ EC shape or CO/sub 2/ Bohr effect. Erythrocyte volume, (metHb), O/sub 2/ binding capacity, and combined red cell organic phosphate concentration (DPG + ATP) were not affected by MIC treatment. However, experimental animals experienced a severe metabolic acid-base disturbance; blood lactate concentration ranged from 8.6 to 24.0 mmole/L. Results indicate that lactic acidosis was solely responsible for increased blood P/sub 50/ of MIC-treated animals. No direct effects of MIC on hemoglobin function were observed. Reduced Hb-O/sub 2/ affinity, in conjunction with severe hypoxemia, compromised the guinea pigs' capacity for pulmonary O/sub 2/ loading; at PaO/sub 2/ of 30 mm Hg, Hb-O/sub 2/ saturation (S) decreased from 66% S for controls to 42% S for MIC-treated animals.

Research Organization:
Brown Univ., Providence, RI
OSTI ID:
6061099
Journal Information:
Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 72; ISSN EVHPA
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ADULTS
AGE GROUPS
ANIMALS
ANOXIA
BIOLOGICAL EFFECTS
BLOOD CHEMISTRY
BLOOD PRESSURE
BODY
CARBOXYLIC ACIDS
ELEMENTS
GLOBIN
GUINEA PIGS
HEMATOLOGY
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HOMEOSTASIS
INHALATION
INTAKE
ISOCYANATES
LUNGS
MAMMALS
MEDICINE
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXYGEN
PHYSIOLOGY
PIGMENTS
PORPHYRINS
PROTEINS
RESPIRATORY SYSTEM
RODENTS
UPTAKE
VERTEBRATES