Biological effects of short-term, high-concentration exposure to methyl isocyanate. IV. Influence on the oxygen binding properties of guinea pig blood
Whole blood oxygen equilibrium curves (O/sub 2/ECs), blood buffer lines, and several hematologic properties were determined for adult guinea pigs exposed to 700 ppm methyl isocyanate (MIC) for 15 min. MIC inhalation effected a significant reduction of blood O/sub 2/ affinity; the half-saturation pressure (P/sub 50/) at 38/sup 0/C increased from the control (untreated) level of 22.8 +/- 0.1 mm Hg to values ranging from 28.5 to 43.7 mm Hg for experimental animals. MIC exposure had no apparent influence on O/sub 2/ EC shape or CO/sub 2/ Bohr effect. Erythrocyte volume, (metHb), O/sub 2/ binding capacity, and combined red cell organic phosphate concentration (DPG + ATP) were not affected by MIC treatment. However, experimental animals experienced a severe metabolic acid-base disturbance; blood lactate concentration ranged from 8.6 to 24.0 mmole/L. Results indicate that lactic acidosis was solely responsible for increased blood P/sub 50/ of MIC-treated animals. No direct effects of MIC on hemoglobin function were observed. Reduced Hb-O/sub 2/ affinity, in conjunction with severe hypoxemia, compromised the guinea pigs' capacity for pulmonary O/sub 2/ loading; at PaO/sub 2/ of 30 mm Hg, Hb-O/sub 2/ saturation (S) decreased from 66% S for controls to 42% S for MIC-treated animals.
- Research Organization:
- Brown Univ., Providence, RI
- OSTI ID:
- 6061099
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 72
- Country of Publication:
- United States
- Language:
- English
Similar Records
Biological effects of short-term, high-concentration exposure to methyl isocyanate. II. Blood chemistry and hematologic evaluations
Effects of carbon dioxide and pH variations in vitro on blood respiratory functions, red blood cell volume, transmembrane pH gradients, and sickling in sickle cell anemia
Related Subjects
ISOCYANATES
BIOLOGICAL EFFECTS
INHALATION
OXYGEN
BLOOD CHEMISTRY
UPTAKE
ACUTE EXPOSURE
ADULTS
ANOXIA
BLOOD PRESSURE
GUINEA PIGS
HEMATOLOGY
HEMOGLOBIN
HOMEOSTASIS
LUNGS
PHYSIOLOGY
AGE GROUPS
ANIMALS
BODY
CARBOXYLIC ACIDS
ELEMENTS
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTAKE
MAMMALS
MEDICINE
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PIGMENTS
PORPHYRINS
PROTEINS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology