Scintigraphic detection of overexpressed c-erbB-2 protooncogene products by a class-switched murine anti-c-erbB-2 protein monoclonal antibody

Journal Article · Fri Feb 01 00:00:00 EST 1991 · Cancer Research; (USA)

OSTI ID:6060124

Saga, T; Endo, K; Akiyama, T; Sakahara, H; Koizumi, M; Watanabe, Y; Nakai, T; Hosono, M; Yamamoto, T; Toyoshima, K ^[1]

---

1. Kyoto Univ. School of Medicine (Japan)

Class-switched monoclonal antibody SV2-61r recognized the extracellular domain of c-erbB-2 protooncogene products separate from the epidermal growth factor receptor. We studied the potential of SV2-61r for evaluating the amplification of c-erbB-2 protooncogene on cancer cells, which has been reported to have prognostic value in adenocarcinoma patients. Radiolabeled SV2-61r specifically bound to various adenocarcinoma cells in addition to c-erbB-2-transfected NIH-3T3 cells (A4) with the affinity constant of 4.4 x 10(8) M-1. SV2-61r injected i.v. localized well to A4 cells xenografted in nude mice. Tumor uptake and localization index of radioiodinated SV2-61r were lower than those of 111In-labeled SV2-61r, probably due to the internalization and dehalogenation of formed antibody-antigen complexes. Biodistribution and specificity of targeting were assessed by comparison among three cells, A4, lung cancer SBC-3 (c-erbB-2 weakly positive) and B-lymphoblastoid Manca cells (c-erbB-2 negative). Tumor:blood ratios, obtained 48 h after injection, were 5.63, 1.45, and 0.68, respectively, indicating the potential of 111In-labeled SV2-61r for evaluating the amplification of c-erbB-2 protooncogene on cancer cells. Because of its close relationship with carcinogenesis and the uniform expression, c-erbB-2 protooncogene products seem to be the optimal target of imaging and therapy of adenocarcinoma patients.

• OSTI ID: 6060124
• Journal Information: Cancer Research; (USA), Vol. 51:3; ISSN 0008-5472
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CARCINOMAS
DIAGNOSIS
GROWTH FACTORS
RECEPTORS
EPIDERMIS
EXPERIMENTAL NEOPLASMS
INDIUM 111
MICE
MONOCLONAL ANTIBODIES
ONCOGENES
RADIOIMMUNOSCINTIGRAPHY
TUMOR CELLS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
GENES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
MINUTES LIVING RADIOISOTOPES
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOIMMUNODETECTION
RADIOISOTOPE SCANNING
RADIOISOTOPES
RODENTS
SCINTISCANNING
SKIN
TISSUES
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics