Amonia gas: an improved reagent for chemical ionization mass spectrometry of bile acid methyl ester acetates
The ammonia chemical ionization mass spectra of 28 methyl ester acetate derivatives of bile acids and related compounds have been determined by gas-liquid chromatography-mass spectrometry. Advantages of ammonia ionization over the previously studied isobutane ionization include a 130 to 270% enhancement in the sensitivity of base peak monitoring, and direct determination of molecular weight from the base peak (M + NH/sub 4//sup +/) in the mass spectrum of any of the derivatives. Minor ions in the ammonia spectra also allow selective detection of 3-keto compounds and can indicate unsaturation or double bond conjugation in the molecule. The significance of these studies for the detection and quantitation of bile acids is discussed. 2 tables.
- Research Organization:
- Argonne National Lab., IL
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 6060057
- Journal Information:
- J. Lipid Res.; (United States), Journal Name: J. Lipid Res.; (United States) Vol. 22; ISSN JLPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400102* -- Chemical & Spectral Procedures
400104 -- Spectral Procedures-- (-1987)
AMMONIA
BILE ACIDS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
GAS CHROMATOGRAPHY
HYDRIDES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
IONIZATION
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
SEPARATION PROCESSES
SPECTROSCOPY
STEROIDS
STEROLS